Paris/August 16
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The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size. | The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size. | ||
| + | |||
| + | |||
| + | == Digestion reactions == | ||
| + | |||
| + | The following digestion reactions (ON) were performed: | ||
| + | |||
| + | <br> | ||
| + | <font color="#FF99FF" size="4">PURPLE = Vector </font><br> | ||
| + | <font color="#ccffcc" size="4">GREEN = Insert </font><br> | ||
| + | {| | ||
| + | |- style="background: #ccccff;" | ||
| + | ! colspan="5" style="background: #ccccff;" | Digestion Products | ||
| + | |- style="background: #ccccff; text-align: center;" | ||
| + | |width=5%| Number | ||
| + | |width=24%| Product Name | ||
| + | |width=20%| Matrix Name | ||
| + | |width=5%| Enzyme 1 | ||
| + | |width=5%| Enzyme 2 | ||
| + | |width=5%| Size | ||
| + | |width=34%| Description | ||
| + | |width=1%| | ||
| + | |width=1%| | ||
| + | |width=5%| Date | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #FF99FF;" |D1 | ||
| + | |<bbpart>pJ23100</bbpart> | ||
| + | |<bbpart>pJ23100</bbpart> | ||
| + | |Spe1 | ||
| + | |Pst1 | ||
| + | |2096 | ||
| + | |Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | ||
| + | | | ||
| + | | | ||
| + | |Aug. 16 | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #FF99FF;" |D3 | ||
| + | |<bbpart>pJ23107</bbpart> | ||
| + | |<bbpart>pJ23107</bbpart> | ||
| + | |Spe1 | ||
| + | |Pst1 | ||
| + | |2096 | ||
| + | |Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | ||
| + | | | ||
| + | | | ||
| + | |Aug. 16 | ||
| + | |- style="background: #cccccc;" | ||
| + | | style="background: #ccffcc;" |D37 | ||
| + | |lox71-gfp(tripart) BI | ||
| + | |L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction) | ||
| + | |XbaI | ||
| + | |PstI | ||
| + | | | ||
| + | |lox71_RBS-gfp-double terminator extracted as a backward insert | ||
| + | | | ||
| + | | | ||
| + | |Aug. 16 | ||
| + | |} | ||
Revision as of 00:30, 17 August 2007
Colony PCRs
The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size.
Digestion reactions
The following digestion reactions (ON) were performed:
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Date | ||
| D1 | pJ23100 | pJ23100 | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | Aug. 16 | ||
| D3 | pJ23107 | pJ23107 | Spe1 | Pst1 | 2096 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | Aug. 16 | ||
| D37 | lox71-gfp(tripart) BI | L12.2 miniprep products (from 30/07 ligation reaction) | XbaI | PstI | lox71_RBS-gfp-double terminator extracted as a backward insert | Aug. 16 | |||
