Paris/August 16

From 2007.igem.org

(Difference between revisions)
(Digestion reactions)
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The following digestion reactions (ON) were performed:
The following digestion reactions (ON) were performed:
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<font color="#FF99FF" size="4">PURPLE = Vector </font><br>
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<font color="#ccffcc" size="4">GREEN = Insert </font><br>
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Digestion Products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=24%| Product Name
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|width=20%| Matrix Name
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|width=5%| Enzyme 1
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|width=5%| Enzyme 2
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|width=5%| Size
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|width=34%| Description
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|width=1%|
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|width=1%|
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|width=5%| Date
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |D37
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|lox71-gfp(tripart) BI
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|L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
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|XbaI
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|PstI
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|
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|lox71_RBS-gfp-double terminator extracted as a backward insert
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|
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|
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|Aug. 16
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|}
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I was unable to perform the following digestion reactions (because their was no more MP1 or MP2 product left):
<br>
<br>
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|Aug. 16
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|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D3
| style="background: #FF99FF;" |D3
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|Aug. 16
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D37
 
-
|lox71-gfp(tripart) BI
 
-
|L12.2 miniprep products (from [[Paris/July 30| 30/07]] ligation reaction)
 
-
|XbaI
 
-
|PstI
 
|
|
-
|lox71_RBS-gfp-double terminator extracted as a backward insert
 
-
|
 
-
|
 
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|Aug. 16
 
|}
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Revision as of 01:17, 17 August 2007

yesterday -- tomorrow

Colony PCRs

The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size.


Digestion reactions

The following digestion reactions (ON) were performed:


PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Date
D37 lox71-gfp(tripart) BI L12.2 miniprep products (from 30/07 ligation reaction) XbaI PstI lox71_RBS-gfp-double terminator extracted as a backward insert Aug. 16



I was unable to perform the following digestion reactions (because their was no more MP1 or MP2 product left):


PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Date
D1 pJ23100 pJ23100 Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
D3 pJ23107 pJ23107 Spe1 Pst1 2096 Vector with the J23107 promoter ready for insertion of a RBS/CDS insert