Paris/August 16
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Revision as of 01:17, 17 August 2007 by Eismoustique (Talk | contribs)
Colony PCRs
The cloning finally seem to have worked, so we are doing colony PCRs to check if there is an insert and if it's got the right size.
Digestion reactions
The following digestion reactions (ON) were performed:
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Date | ||
| D37 | lox71-gfp(tripart) BI | L12.2 miniprep products (from 30/07 ligation reaction) | XbaI | PstI | lox71_RBS-gfp-double terminator extracted as a backward insert | Aug. 16 | |||
I was unable to perform the following digestion reactions (because their was no more MP1 or MP2 product left):
PURPLE = Vector
GREEN = Insert
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Date | ||
| D1 | pJ23100 | pJ23100 | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | |||
| D3 | pJ23107 | pJ23107 | Spe1 | Pst1 | 2096 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | |||
