Paris/August 2

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DGAT Assembly PCR: Megaprimer PCR

  • Material:

- DGAT deltaPst-1: 7µL (35ng)
- Tampon: 10µL
- dNTP: 1µL (final 200µmol each)
- Taq: 0.5µL
- Water: 29µL
- Primer1: ForDGATbb: 2.5µL (final 0.5µM)
- Primer2: RevDGATbb: 2.5µL (final 0.5µM)

[[Image: |right|100px]]

  • PCR Program:

5 cycles only with DGAT2 as primer:
- 95°C: 5min
- 95°C: 1min (X35)
- 55°C: 30s (X35)
- 72°C: 2min (X35)
- 72°C: 10 min
- 4°C: inf

  • Quantity: µg/µL
  • Expected size: 1443 bp
  • After purification:

2µL of purification product (2µL of ladder)

100px
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