Paris/August 21

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< Paris(Difference between revisions)
(Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow)
 
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[[Paris/August 20|yesterday]] -- [[Paris/August 22|tomorrow]]
[[Paris/August 20|yesterday]] -- [[Paris/August 22|tomorrow]]
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== Growth kinetic on w121 strains ==
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== Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow ==
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{{Paris_KineticArray| Title = w121 kinetic as a function of DAP and supplemented medium (S0.x)
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We spread LB with W121 (Erythromycine; DAP) and FX85 (Kanamycine; Thymine)
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|A1=H<sub>2</sub>0|A2=H<sub>2</sub>0|A3=H<sub>2</sub>0|A4=H<sub>2</sub>0
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|A5=H<sub>2</sub>0|A6=H<sub>2</sub>0|A7=H<sub>2</sub>0|A8=H<sub>2</sub>0
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== Culture of Ligation Strains ==
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|A9=H<sub>2</sub>0|A10=H<sub>2</sub>0|A11=H<sub>2</sub>0|A12=H<sub>2</sub>0
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|B1=H<sub>2</sub>0
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We made 5mL culture of the new ligation strains for future minipreps and glycerol stocks.<br>
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|B2=LB+0µM DAP|B3=LB+5µM DAP|B4=LB+8µM DAP|B5=LB+10µM DAP|B6=LB+12µM DAP
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Here are the Ligation strains put in culture.<br>
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|B7=LB+15µM DAP|B8=LB+17µM DAP|B9=LB+20µM DAP|B10=LB+30µM DAP|B11=LB+40µM DAP
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|B12=H<sub>2</sub>0|C1=H<sub>2</sub>0
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*L31.1 - .3 - .4
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|C2=S0.2+0µM DAP|C3=S0.2+5µM DAP|C4=S0.2+6µM DAP|C5=S0.2+7µM DAP|C6=S0.2+8µM DAP
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*L32.1 - .2 - .3
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|C7=S0.2+9µM DAP|C8=S0.2+10µM DAP|C9=S0.2+15µM DAP|C10=S0.2+17µM DAP|C11=S0.2+20µM DAP
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*L34.1 - .2 - .3
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|C12=H<sub>2</sub>0|D1=H<sub>2</sub>0
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*L35.1 - .2 - .3
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|D2=S0.4+0µM DAP|D3=S0.4+2µM DAP|D4=S0.4+3µM DAP|D5=S0.4+4µM DAP|D6=S0.4+5µM DAP
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*L36.1 - .2 - .3
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|D7=S0.4+8µM DAP|D8=S0.4+10µM DAP|D9=S0.4+12µM DAP|D10=S0.4+15µM DAP|D11=S0.4+20µM DAP
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*L37.1 - .2 - .3
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|D12=H<sub>2</sub>0|E1=H<sub>2</sub>0
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*L39.1 - .2 - .3
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|E2=S0.6+0µM DAP|E3=S0.6+1µM DAP|E4=S0.6+2µM DAP|E5=S0.6+3µM DAP|E6=S0.6+4µM DAP
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*L40.1 - .2
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|E7=S0.6+5µM DAP|E8=S0.6+6µM DAP|E9=S0.6+7µM DAP|E10=S0.6+8µM DAP|E11=S0.6+10µM DAP
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*L41.1
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|E12=H<sub>2</sub>0|F1=H<sub>2</sub>0
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*L42.1 - .2 - .3
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|F2=S0.8+0µM DAP|F3=S0.8+0,5µM DAP|F4=S0.8+1µM DAP|F5=S0.8+2µM DAP|F6=S0.8+3µM DAP
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*L43.1 - .2 - .3
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|F7=S0.8+4µM DAP|F8=S0.8+5µM DAP|F9=S0.8+6µM DAP|F10=S0.8+7µM DAP|F11=S0.8+10µM DAP
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|F12=H<sub>2</sub>0|G1=H<sub>2</sub>0
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|G2=H<sub>2</sub>0|G3=H<sub>2</sub>0|G4=H<sub>2</sub>0|G5=H<sub>2</sub>0|G6=H<sub>2</sub>0
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|G7=H<sub>2</sub>0|G8=H<sub>2</sub>0|G9=H<sub>2</sub>0|G10=H<sub>2</sub>0|G11=H<sub>2</sub>0
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|G12=H<sub>2</sub>0|H1=H<sub>2</sub>0|H2=H<sub>2</sub>0|H3=H<sub>2</sub>0|H4=H<sub>2</sub>0
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|H5=H<sub>2</sub>0|H6=H<sub>2</sub>0|H7=H<sub>2</sub>0|H8=H<sub>2</sub>0
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|H9=H<sub>2</sub>0|H10=H<sub>2</sub>0|H11=H<sub>2</sub>0|H12=H<sub>2</sub>0}}
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Latest revision as of 13:27, 27 August 2007

yesterday -- tomorrow

Culture of w121 and FX85 strains for preparing chemiocompetent cells on tomorrow

We spread LB with W121 (Erythromycine; DAP) and FX85 (Kanamycine; Thymine)

Culture of Ligation Strains

We made 5mL culture of the new ligation strains for future minipreps and glycerol stocks.
Here are the Ligation strains put in culture.

  • L31.1 - .3 - .4
  • L32.1 - .2 - .3
  • L34.1 - .2 - .3
  • L35.1 - .2 - .3
  • L36.1 - .2 - .3
  • L37.1 - .2 - .3
  • L39.1 - .2 - .3
  • L40.1 - .2
  • L41.1
  • L42.1 - .2 - .3
  • L43.1 - .2 - .3