Paris/August 22

From 2007.igem.org

(Difference between revisions)
(Minipreps of Ligation transformation strains from yesterday)
Line 2: Line 2:
== Minipreps of Ligation transformation strains from yesterday ==
== Minipreps of Ligation transformation strains from yesterday ==
 +
 +
*L31.1 - .3 - .4
 +
*L32.1 - .2 - .3
 +
*L34.1 - .2 - .3
 +
*L35.1 - .2 - .3
 +
*L36.1 - .2 - .3
 +
*L37.1 - .2 - .3
 +
*L39.1 - .2 - .3
 +
*L40.1 - .2
 +
*L41.1
 +
*L42.1 - .2 - .3
 +
*L43.1 - .2 - .3
 +
== Growth kinetic on w121 strains ==
== Growth kinetic on w121 strains ==

Revision as of 16:13, 21 August 2007

yesterday -- tomorrow

Contents

Minipreps of Ligation transformation strains from yesterday

  • L31.1 - .3 - .4
  • L32.1 - .2 - .3
  • L34.1 - .2 - .3
  • L35.1 - .2 - .3
  • L36.1 - .2 - .3
  • L37.1 - .2 - .3
  • L39.1 - .2 - .3
  • L40.1 - .2
  • L41.1
  • L42.1 - .2 - .3
  • L43.1 - .2 - .3

Growth kinetic on w121 strains

Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x)
1 2 3 4 5 6 7 8 9 10 11 12
A H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
B H20 LB+0µM DAP LB+5µM DAP LB+8µM DAP LB+10µM DAP LB+12µM DAP LB+15µM DAP LB+17µM DAP LB+20µM DAP LB+30µM DAP LB+40µM DAP H20
C H20 S0.2+0µM DAP S0.2+5µM DAP S0.2+6µM DAP S0.2+7µM DAP S0.2+8µM DAP S0.2+9µM DAP S0.2+10µM DAP S0.2+15µM DAP S0.2+17µM DAP S0.2+20µM DAP H20
D H20 S0.4+0µM DAP S0.4+2µM DAP S0.4+3µM DAP S0.4+4µM DAP S0.4+5µM DAP S0.4+8µM DAP S0.4+10µM DAP S0.4+12µM DAP S0.4+15µM DAP S0.4+20µM DAP H20
E H20 S0.6+0µM DAP S0.6+1µM DAP S0.6+2µM DAP S0.6+3µM DAP S0.6+4µM DAP S0.6+5µM DAP S0.6+6µM DAP S0.6+7µM DAP S0.6+8µM DAP S0.6+10µM DAP H20
F H20 S0.8+0µM DAP S0.8+0,5µM DAP S0.8+1µM DAP S0.8+2µM DAP S0.8+3µM DAP S0.8+4µM DAP S0.8+5µM DAP S0.8+6µM DAP S0.8+7µM DAP S0.8+10µM DAP H20
G H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
H H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20

Preparing chemically competent cells

  • w121
  • FX85

CRE Recombination test

DapAp expression test