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		+	[[Paris/August 23|yesterday]] -- [[Paris/August 25|tomorrow]]
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	== Recombination Rate Test ==		== Recombination Rate Test ==

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Revision as of 14:21, 27 August 2007

yesterday -- tomorrow

Recombination Rate Test

We obtained clones on every plate ! cool. Our competent bacteria were well competent...
Then we took 100 FX85 clones and we put them on AMP-KAN-THYMINE.
We dillute to 10-7 our liquid culture and then we spread 100µL onto AMP-THYMINE plates

Transformation of W121 with DapAp-mRFP constructrion

We obtained clones from our last transformation. We took one clone from the plate with L26.1 transformation and 1 from L26.2 transformation.
Then we spread in 5mL of LB those clones with Amp+erythro+DAP.