Paris/August 3
From 2007.igem.org
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[[Image: DGATbbdigEcoR1Pst108032007.jpg|100px|right]] | [[Image: DGATbbdigEcoR1Pst108032007.jpg|100px|right]] | ||
| - | The double digestion | + | The double digestion allows us to insert the biobrick into biobrick plasmid.<br> |
| - | The Pst1 digestion | + | The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested). |
Revision as of 14:04, 3 August 2007
DGAT biobrick: digestion, cloning
* EcoR1-Pst1 digestion
The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).
