Paris/August 3

From 2007.igem.org

(Difference between revisions)
(TOPO cloning)
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== TOPO cloning ==
== TOPO cloning ==
-
*P7
+
{|
-
*P7
+
|- style="background: #ccccff;"
-
*P8
+
! colspan="5" style="background: #ccccff;" | PCR products
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=10%| Name
 +
|width=15%| Oligo F
 +
|width=15%| Oligo R
 +
|width=10%| Matrix
 +
|width=5%| Length
 +
|width=25%| Comments
 +
|width=5%| Product Left ?
 +
|width=5%| Date
 +
|[[Paris/July 17| July 17]], [[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |P6
 +
|Lox66-DapASubtilis
 +
|O8 Lox66-DapASubt-F
 +
|O9 DapASubt-R
 +
|B.Subtilis 168
 +
|979
 +
|
 +
|
 +
|[[Paris/July 17| July 17]],[[Paris/July 27| July 27]],[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |P7
 +
|Lox71-FtsZ
 +
| O1 Lox71-FtsZ-F
 +
| O2 FtsZ-R
 +
|MG1655
 +
|1260
 +
|EcoRI site in FtsZ not mutated
 +
|
 +
|[[Paris/July 17| July 17]],[[Paris/July 30| July 30]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |P8
 +
|RBS-DapAColi
 +
| O20 RBS-DapAColi-F
 +
| O7 DapAColi-R
 +
| MG1655
 +
|952
 +
|B0030-DapAColi
 +
|
 +
|}

Revision as of 15:55, 3 August 2007

DGAT biobrick: digestion, cloning

  • EcoR1-Pst1 digestion
DGATbbdigEcoR1Pst108032007.jpg

The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).

TOPO cloning

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? Date July 17, July 30
P6 Lox66-DapASubtilis O8 Lox66-DapASubt-F O9 DapASubt-R B.Subtilis 168 979 July 17, July 27, July 30
P7 Lox71-FtsZ O1 Lox71-FtsZ-F O2 FtsZ-R MG1655 1260 EcoRI site in FtsZ not mutated July 17, July 30
P8 RBS-DapAColi O20 RBS-DapAColi-F O7 DapAColi-R MG1655 952 B0030-DapAColi