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(TOPO cloning)
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== TOPO cloning ==
== TOPO cloning ==
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Cloning using TOPO protocol of the following purification of PCR products<br>
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Revision as of 16:00, 3 August 2007

DGAT biobrick: digestion, cloning

  • EcoR1-Pst1 digestion
DGATbbdigEcoR1Pst108032007.jpg

The double digestion allows us to insert the biobrick into biobrick plasmid.
The Pst1 digestion allows us to see if the mutagenesis worked (dgat gene not digested).

TOPO cloning

Cloning using TOPO protocol of the following purification of PCR products

Number Name Oligo F Oligo R Matrix Length Comments
P6 Lox66-DapASubtilis O8 Lox66-DapASubt-F O9 DapASubt-R B.Subtilis 168 979
P7 Lox71-FtsZ O1 Lox71-FtsZ-F O2 FtsZ-R MG1655 1260 EcoRI site in FtsZ not mutated
P8 RBS-DapAColi O20 RBS-DapAColi-F O7 DapAColi-R MG1655 952 B0030-DapAColi