Paris/August 31

From 2007.igem.org

yesterday -- tomorrow

Growth kinetic on w121 strains

In order to precise the growth behavior/kinetics of our strain W121 in smaller DAP concentration, we fit those last ones in function of our last kinetics results (06/08/07).
But we will use this experiment to better understand the presence of the delay observed in low DAP conentration.

Kinetic Array :w121 kinetic as a function of DAP and supplemented medium (S0.x)
1 2 3 4 5 6 7 8 9 10 11 12
A H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
B H20 LB+0µM DAP LB+5µM DAP LB+8µM DAP LB+10µM DAP LB+12µM DAP LB+15µM DAP LB+17µM DAP LB+20µM DAP LB+30µM DAP LB+40µM DAP H20
C H20 S0.2+0µM DAP S0.2+5µM DAP S0.2+6µM DAP S0.2+7µM DAP S0.2+8µM DAP S0.2+9µM DAP S0.2+10µM DAP S0.2+15µM DAP S0.2+17µM DAP S0.2+20µM DAP H20
D H20 S0.4+0µM DAP S0.4+2µM DAP S0.4+3µM DAP S0.4+4µM DAP S0.4+5µM DAP S0.4+8µM DAP S0.4+10µM DAP S0.4+12µM DAP S0.4+15µM DAP S0.4+20µM DAP H20
E H20 S0.6+0µM DAP S0.6+1µM DAP S0.6+2µM DAP S0.6+3µM DAP S0.6+4µM DAP S0.6+5µM DAP S0.6+6µM DAP S0.6+7µM DAP S0.6+8µM DAP S0.6+10µM DAP H20
F H20 S0.8+0µM DAP S0.8+0,5µM DAP S0.8+1µM DAP S0.8+2µM DAP S0.8+3µM DAP S0.8+4µM DAP S0.8+5µM DAP S0.8+6µM DAP S0.8+7µM DAP S0.8+10µM DAP H20
G H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20
H H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20 H20

The 96 well plate is surrounded by H2O in order to maintain humidity during the assay. In each slot, 200µL of the growth medium (LB or S0.x) is mixed with 2µL of w121 culture grown ON, and with different amount of DAP (see table). The growth profile is measured over a period of 20H, a DO measurement is acquired each 4min10s.

In order to determine whether the delay observed would come from the growth of a mutant, we'll retake the broth with a 100X dilution. Will this delay appear again and the same way as first observed ?