Paris/August 7

From 2007.igem.org

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Revision as of 18:18, 7 August 2007

Study of the pBAD promoter

We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI)


Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D37'.1 lox71-gfp(tripart) BI L12.1 miniprep product (from 30/07 ligation reaction) XbaI PstI lox71_RBS-rfp-double terminator extracted as a backward insert
D39' BI-insert lox66-DapAE.coli lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) XbaI PstI lox66-dapAcoli extracted for usage as a backward insert
D41 araC/pBad-J61002 plasmid SP dig. L1.2, L1".1 & L1".2 miniprep product SpeI PstI J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS

Minipreps

Number Name Description Products in use Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2) July 15