Paris/August 7

From 2007.igem.org

(Difference between revisions)
(Minipreps)
(Minipreps)
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|width=50%| Description
|width=50%| Description
|width=15%| Products in use
|width=15%| Products in use
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|width=5%| Date
 
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP1
| style="background: #ccffcc;" |MP1
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|Strong constitutive promoter in J61002 (clones 1 + 2)
|Strong constitutive promoter in J61002 (clones 1 + 2)
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|
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| [[Paris/July 15| July 15]]
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|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |MP1
 +
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|}

Revision as of 18:21, 7 August 2007

Study of the pBAD promoter

We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI)


Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D37'.1 lox71-gfp(tripart) BI L12.1 miniprep product (from 30/07 ligation reaction) XbaI PstI lox71_RBS-rfp-double terminator extracted as a backward insert
D39' BI-insert lox66-DapAE.coli lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) XbaI PstI lox66-dapAcoli extracted for usage as a backward insert
D41 araC/pBad-J61002 plasmid SP dig. L1.2, L1".1 & L1".2 miniprep product SpeI PstI J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS

Minipreps

Number Name Description Products in use
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2)