Paris/August 7

From 2007.igem.org

(Difference between revisions)
(Minipreps)
(Minipreps)
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|width=15%| Products in use
|width=15%| Products in use
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT2
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|S16.2
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT3.1
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P6.1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT3.2
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P6.7
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT4.1
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P8.2
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT4.2
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P8.5
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT4.3
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P8.7
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT4.4
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P8.8
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT4.5
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P8.10
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT5.1
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P9.1
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT5.2
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P9.2
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
-
| style="background: #ccffcc;" |MP1
+
| style="background: #ccffcc;" |MPT5.3
-
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart>
+
|
-
|Strong constitutive promoter in J61002 (clones 1 + 2)
+
|pTOPO P9.4
|
|
|}
|}

Revision as of 18:31, 7 August 2007

Study of the pBAD promoter

We've put the AraC-pBAD promoter (BBa_I0500) in the expression vector BBa_J61002 (between EcoRI and SpeI)


Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D37'.1 lox71-gfp(tripart) BI L12.1 miniprep product (from 30/07 ligation reaction) XbaI PstI lox71_RBS-rfp-double terminator extracted as a backward insert
D39' BI-insert lox66-DapAE.coli lox66-DapAE.coli in pTOPO (P5 PCR product)(clones 1+2) XbaI PstI lox66-dapAcoli extracted for usage as a backward insert
D41 araC/pBad-J61002 plasmid SP dig. L1.2, L1".1 & L1".2 miniprep product SpeI PstI J61002 plasmid containing araC/pBad promotor digested for insertion of a BI CDS

Minipreps

Number Name Description Products in use
MPT2 S16.2
MPT3.1 pTOPO P6.1
MPT3.2 pTOPO P6.7
MPT4.1 pTOPO P8.2
MPT4.2 pTOPO P8.5
MPT4.3 pTOPO P8.7
MPT4.4 pTOPO P8.8
MPT4.5 pTOPO P8.10
MPT5.1 pTOPO P9.1
MPT5.2 pTOPO P9.2
MPT5.3 pTOPO P9.4