Paris/Freezer

From 2007.igem.org

(Difference between revisions)
(→Digestion Products)
(→Strains (glycerol stock))
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|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S4
| style="background: #ccffcc;" |S4
-
|pJ23100
+
|<bbpart>BBa_pJ23100</bbpart>
|Strong constitutive promoter in J61002 (clones 1 + 2)
|Strong constitutive promoter in J61002 (clones 1 + 2)
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S5
| style="background: #ccffcc;" |S5
-
|BBa_B0015
+
|<bbpart>BBa_B0015</bbpart>
|Double terminator (clones 1 + 2)
|Double terminator (clones 1 + 2)
|
|
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S6
| style="background: #ccffcc;" |S6
-
|pJ23107
+
|<bbpart>BBa_pJ23107</bbpart>
|Medium constitutive promoter in J61002 (clones 1 + 2)  
|Medium constitutive promoter in J61002 (clones 1 + 2)  
|19/07/07
|19/07/07
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S7
| style="background: #ccffcc;" |S7
-
|BBa_B0030
+
|<bbpart>BBa_B0030</bbpart>
|Strong RBS (clones 1 + 2)
|Strong RBS (clones 1 + 2)
|19/07/07
|19/07/07
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S8
| style="background: #ccffcc;" |S8
-
|BBa_E0422
+
|<bbpart>BBa_E0422</bbpart>
|ECFP (RBS+LVA+Term) (clones 1 + 2)  
|ECFP (RBS+LVA+Term) (clones 1 + 2)  
|19/07/07
|19/07/07
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S9
| style="background: #ccffcc;" |S9
-
|BBa_E0241
+
|<bbpart>BBa_E0241</bbpart>
|PoPs to GFP converter (clones 1 + 2)
|PoPs to GFP converter (clones 1 + 2)
|19/07/07
|19/07/07
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S10
| style="background: #ccffcc;" |S10
-
|BBa_E0840
+
|<bbpart>BBa_E0840</bbpart>
|gfp tri-part; strong rbs (clones 1 + 2)
|gfp tri-part; strong rbs (clones 1 + 2)
|19/07/07
|19/07/07
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S11
| style="background: #ccffcc;" |S11
-
|BBa_J61047
+
|<bbpart>BBa_J61047</bbpart>
|Cre ORF (clones 1 + 2)
|Cre ORF (clones 1 + 2)
|19/07/07
|19/07/07
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|
|
|05/07/07
|05/07/07
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S13
 +
|<bbpart>BBa_I0500</bbpart>
 +
|pBAD AraC (clone 1+2)
 +
|20/07/07
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S14
 +
|<bbpart>BBa_pJ23107</bbpart>
 +
|Medium constitutive promoter in J61002 (clone 3+4)
 +
|20/07/07
|}
|}

Revision as of 12:37, 20 July 2007

Contents

PCR products

PCR products
Number Name Length Comments Product Left ? Date
P1 Lox71-FtsA-FtsZ-1 1537
P2 FtsZ-2 1088
P3 DGAT-1
P4 DGAT-2
P5 Lox66-DapAColi 985
P6 Lox66-DapASubtilis 979 17/07/07
P7 Lox71-FtsZ 1260 EcoR1 site not mutated 17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Product Left ? Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP3 B0015 in pSB1AK3 Double terminator (clones 1 + 2) 16/07/07
MP4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2) 16/07/07
MP5 BBa_B0030 in pSB1A2 Strong RBS (clones 1 + 2) 18/07/07
MP6 BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) 18/07/07
MP7 BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2) 18/07/07
MP8 BBa_E0840 in pSB1A2 gfp tri-part; strong rbs (clones 1 + 2) 18/07/07
MP9 BBa_J61047 in pSB1A2 Cre ORF (clones 1 + 2) 18/07/07

Digestion Products

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Description Product Left Date
D1 pJ23100 pJ23100 Spe1 Pst1 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert 17/07/07
D2 mRFP pJ23100 Spe1 Pst1 17/07/07
D3 pJ23107 pJ23107 Spe1 Pst1 Vector with the J23107 promoter ready for insertion of a RBS/CDS insert 17/07/07
D4 mRFP pJ23107 Spe1 Pst1 17/07/07
D5 pJ23100 pJ23100 EcoR1 Spe1 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 17/07/07
D6 pJ23107 pJ23107 EcoR1 Spe1 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 17/07/07
D7 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) EcoR1 Pst1 17/07/07
D8 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) Xba1 Pst1 17/07/07

Strains (glycerol stock)

Strains glycerol stock
Number Name Description Date
S1 MG1655 Standard E.Coli strain
S2 Acinetobacter ADP1 Strain used for TG coloration test with Red Nile
S3 DH5alpha pKs::DGAT Enzyme catalysing the transormation of DAG into TG
S4 BBa_pJ23100 Strong constitutive promoter in J61002 (clones 1 + 2)
S5 BBa_B0015 Double terminator (clones 1 + 2)
S6 BBa_pJ23107 Medium constitutive promoter in J61002 (clones 1 + 2) 19/07/07
S7 BBa_B0030 Strong RBS (clones 1 + 2) 19/07/07
S8 BBa_E0422 ECFP (RBS+LVA+Term) (clones 1 + 2) 19/07/07
S9 BBa_E0241 PoPs to GFP converter (clones 1 + 2) 19/07/07
S10 BBa_E0840 gfp tri-part; strong rbs (clones 1 + 2) 19/07/07
S11 BBa_J61047 Cre ORF (clones 1 + 2) 19/07/07
S12 W121 05/07/07
S13 BBa_I0500 pBAD AraC (clone 1+2) 20/07/07
S14 BBa_pJ23107 Medium constitutive promoter in J61002 (clone 3+4) 20/07/07


  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
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