Paris/Freezer
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|Strong constitutive promoter in J61002 (clones 1 + 2) | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 15| July 15]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP2 | | style="background: #ccffcc;" |MP2 | ||
Line 138: | Line 138: | ||
|Medium constitutive promoter in J61002 (clones 1 + 2) | |Medium constitutive promoter in J61002 (clones 1 + 2) | ||
|MP2.2 | |MP2.2 | ||
- | | | + | | [[Paris/July 15| July 15]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP3 | | style="background: #ccffcc;" |MP3 | ||
Line 144: | Line 144: | ||
|Double terminator (clones 1 + 2) | |Double terminator (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 16| July 16]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP4 | | style="background: #ccffcc;" |MP4 | ||
Line 150: | Line 150: | ||
|AraC pBAD (clones 1 + 2) | |AraC pBAD (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 16| July 16]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP5 | | style="background: #ccffcc;" |MP5 | ||
Line 156: | Line 156: | ||
|Strong RBS (clones 1 + 2) | |Strong RBS (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 18| July 18]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP6 | | style="background: #ccffcc;" |MP6 | ||
Line 162: | Line 162: | ||
|ECFP (RBS+LVA+Term) (clones 1 + 2) | |ECFP (RBS+LVA+Term) (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 18| July 18]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP6' | | style="background: #ccffcc;" |MP6' | ||
Line 174: | Line 174: | ||
|PoPs to GFP converter (clones 1 + 2) | |PoPs to GFP converter (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 18| July 18]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP7' | | style="background: #ccffcc;" |MP7' | ||
Line 186: | Line 186: | ||
|gfp tri-part; strong rbs (clones 1 + 2) | |gfp tri-part; strong rbs (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 18| July 18]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP9 | | style="background: #ccffcc;" |MP9 | ||
Line 192: | Line 192: | ||
|Cre ORF (clones 1 + 2) | |Cre ORF (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 18| July 18]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP9' | | style="background: #ccffcc;" |MP9' | ||
Line 204: | Line 204: | ||
|AraC pBAD (clones 1 + 2) | |AraC pBAD (clones 1 + 2) | ||
| | | | ||
- | | | + | | [[Paris/July 20| July 20]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP11 | | style="background: #ccffcc;" |MP11 | ||
Line 210: | Line 210: | ||
|Medium constitutive promoter in J61002 (clones 3 + 4) | |Medium constitutive promoter in J61002 (clones 3 + 4) | ||
|MP11.3 | |MP11.3 | ||
- | | | + | | [[Paris/July 20| July 20]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |MP11' | | style="background: #ccffcc;" |MP11' | ||
Line 216: | Line 216: | ||
|Medium constitutive promoter in J61002 (clones 3) | |Medium constitutive promoter in J61002 (clones 3) | ||
| | | | ||
- | | | + | | [[Paris/July 23| July 23]] |
|} | |} | ||
Line 245: | Line 245: | ||
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | |Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D2 | | style="background: #ccffcc;" |D2 | ||
Line 255: | Line 255: | ||
| | | | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D3 | | style="background: #FF99FF;" |D3 | ||
Line 265: | Line 265: | ||
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | |Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D4 | | style="background: #FF99FF;" |D4 | ||
Line 275: | Line 275: | ||
| | | | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D5 | | style="background: #FF99FF;" |D5 | ||
Line 285: | Line 285: | ||
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | |The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D6 | | style="background: #FF99FF;" |D6 | ||
Line 295: | Line 295: | ||
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | |The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D7 | | style="background: #ccffcc;" |D7 | ||
Line 305: | Line 305: | ||
| | | | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D8 | | style="background: #ccffcc;" |D8 | ||
Line 315: | Line 315: | ||
| | | | ||
| | | | ||
- | | | + | |[[Paris/July 17| July 17]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D9 | | style="background: #FF99FF;" |D9 | ||
Line 325: | Line 325: | ||
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | |Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D10 | | style="background: #ccffcc;" |D10 | ||
Line 335: | Line 335: | ||
|J23100 promoter ready for use as a fw-insert | |J23100 promoter ready for use as a fw-insert | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D11 | | style="background: #FF99FF;" |D11 | ||
Line 345: | Line 345: | ||
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | |Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D12 | | style="background: #ccffcc;" |D12 | ||
Line 355: | Line 355: | ||
|J23107 promoter ready for usage as a fw-insert | |J23107 promoter ready for usage as a fw-insert | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D13 | | style="background: #ccffcc;" |D13 | ||
Line 365: | Line 365: | ||
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it | |mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D14 | | style="background: #ccffcc;" |D14 | ||
Line 375: | Line 375: | ||
|Cre ORF extracted for usage as a bw-insert | |Cre ORF extracted for usage as a bw-insert | ||
|Ø D14.1 | |Ø D14.1 | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D15 | | style="background: #FF99FF;" |D15 | ||
Line 385: | Line 385: | ||
|bacward vector for cloning a CDS bw-insert after the B0030 RBS | |bacward vector for cloning a CDS bw-insert after the B0030 RBS | ||
| | | | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D18 | | style="background: #ccffcc;" |D18 | ||
Line 395: | Line 395: | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|Ø | |Ø | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D19 | | style="background: #ccffcc;" |D19 | ||
Line 405: | Line 405: | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
|Ø | |Ø | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D20 | | style="background: #ccffcc;" |D20 | ||
Line 415: | Line 415: | ||
|PCR product digested for usage as a backward insert | |PCR product digested for usage as a backward insert | ||
|Ø | |Ø | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D21 | | style="background: #ccffcc;" |D21 | ||
Line 425: | Line 425: | ||
|PCR product digested for usage as a backward insert | |PCR product digested for usage as a backward insert | ||
|Ø | |Ø | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #FF99FF;" |D22 | | style="background: #FF99FF;" |D22 | ||
Line 435: | Line 435: | ||
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio | |Open pSB1A2 vector for BioBrick EcoRI/PstI insertio | ||
|D22.1 Ø | |D22.1 Ø | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D23 | | style="background: #ccffcc;" |D23 | ||
Line 445: | Line 445: | ||
|AraC/pBad promoter ready for use as a forward insert | |AraC/pBad promoter ready for use as a forward insert | ||
|Ø D23.1 | |Ø D23.1 | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D24 | | style="background: #ccffcc;" |D24 | ||
Line 455: | Line 455: | ||
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert | |eCFP (RBS-OFR_LVA-T) ready for use as a backward insert | ||
| | | | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D25 | | style="background: #ccffcc;" |D25 | ||
Line 465: | Line 465: | ||
|PoPs to GFP converter ready for use as a backward insert | |PoPs to GFP converter ready for use as a backward insert | ||
| | | | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D26 | | style="background: #ccffcc;" |D26 | ||
Line 475: | Line 475: | ||
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert | |gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert | ||
| | | | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D27 | | style="background: #ccffcc;" |D27 | ||
Line 485: | Line 485: | ||
|lox71-ftsZ(unmutated) ready for use as a backward insert | |lox71-ftsZ(unmutated) ready for use as a backward insert | ||
|Ø | |Ø | ||
- | | | + | |[[Paris/July 23| July 23]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D28 | | style="background: #ccffcc;" |D28 | ||
Line 495: | Line 495: | ||
|Biobrick | |Biobrick | ||
| | | | ||
- | | | + | |[[Paris/July 25| July 25]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D29 | | style="background: #ccffcc;" |D29 | ||
Line 505: | Line 505: | ||
|Backward insert | |Backward insert | ||
| | | | ||
- | | | + | |[[Paris/July 25| July 25]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D30 | | style="background: #ccffcc;" |D30 | ||
Line 515: | Line 515: | ||
|Biobrick | |Biobrick | ||
| | | | ||
- | | | + | |[[Paris/July 25| July 25]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D31 | | style="background: #ccffcc;" |D31 | ||
Line 525: | Line 525: | ||
|Backward insert | |Backward insert | ||
| | | | ||
- | | | + | |[[Paris/July 25| July 25]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D32 | | style="background: #ccffcc;" |D32 | ||
Line 535: | Line 535: | ||
|Backward insert - EcoRI site still presents : we can't use EcoRI | |Backward insert - EcoRI site still presents : we can't use EcoRI | ||
| | | | ||
- | | | + | |[[Paris/July 25| July 25]] |
|} | |} | ||
Line 557: | Line 557: | ||
|~3000 | |~3000 | ||
|We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3 | |We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3 | ||
- | | | + | |[[Paris/July 21| July 21]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D17 | | style="background: #ccffcc;" |D17 | ||
Line 565: | Line 565: | ||
|~2040 + ~920 | |~2040 + ~920 | ||
|3 bands | |3 bands | ||
- | | | + | |[[Paris/July 21| July 21]] |
|} | |} | ||
Line 613: | Line 613: | ||
|Medium constitutive promoter in J61002 (clones 1 + 2) | |Medium constitutive promoter in J61002 (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S7 | | style="background: #ccffcc;" |S7 | ||
Line 619: | Line 619: | ||
|Strong RBS (clones 1 + 2) | |Strong RBS (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S8 | | style="background: #ccffcc;" |S8 | ||
Line 625: | Line 625: | ||
|ECFP (RBS+LVA+Term) (clones 1 + 2) | |ECFP (RBS+LVA+Term) (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S9 | | style="background: #ccffcc;" |S9 | ||
Line 631: | Line 631: | ||
|PoPs to GFP converter (clones 1 + 2) | |PoPs to GFP converter (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S10 | | style="background: #ccffcc;" |S10 | ||
Line 637: | Line 637: | ||
|gfp tri-part; strong rbs (clones 1 + 2) | |gfp tri-part; strong rbs (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S11 | | style="background: #ccffcc;" |S11 | ||
Line 643: | Line 643: | ||
|Cre ORF (clones 1 + 2) | |Cre ORF (clones 1 + 2) | ||
| | | | ||
- | | | + | |[[Paris/July 19| July 19]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S12 | | style="background: #ccffcc;" |S12 | ||
Line 655: | Line 655: | ||
|pBAD AraC (clone 1+2) | |pBAD AraC (clone 1+2) | ||
| | | | ||
- | | | + | |[[Paris/July 20| July 20]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S14 | | style="background: #ccffcc;" |S14 | ||
Line 661: | Line 661: | ||
|Medium constitutive promoter in J61002 (clone 3+4) | |Medium constitutive promoter in J61002 (clone 3+4) | ||
| | | | ||
- | | | + | |[[Paris/July 20| July 20]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S15 | | style="background: #ccffcc;" |S15 | ||
Line 667: | Line 667: | ||
|Kanamycin resistance cassette (clone 1+2) | |Kanamycin resistance cassette (clone 1+2) | ||
| | | | ||
- | | | + | |[[Paris/July 20| July 20]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S16 | | style="background: #ccffcc;" |S16 | ||
Line 673: | Line 673: | ||
|AraC pBad regulating mRFP expression in pJ61002 (L1) | |AraC pBad regulating mRFP expression in pJ61002 (L1) | ||
| | | | ||
- | | | + | |[[Paris/July 27| July 27]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S17 | | style="background: #ccffcc;" |S17 | ||
Line 679: | Line 679: | ||
|Lox66-DapA Coli cloned as a biobrick (L2) | |Lox66-DapA Coli cloned as a biobrick (L2) | ||
| | | | ||
- | | | + | |[[Paris/July 27| July 27]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S18 | | style="background: #ccffcc;" |S18 | ||
Line 685: | Line 685: | ||
|RBS(B0030)-Cre ORF (L6) | |RBS(B0030)-Cre ORF (L6) | ||
| | | | ||
- | | | + | |[[Paris/July 27| July 27]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S19 | | style="background: #ccffcc;" |S19 | ||
Line 691: | Line 691: | ||
|Lox66 (L9) | |Lox66 (L9) | ||
| | | | ||
- | | | + | |[[Paris/July 27| July 27]] |
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |S20 | | style="background: #ccffcc;" |S20 | ||
Line 697: | Line 697: | ||
|Lox71 (L10) | |Lox71 (L10) | ||
| | | | ||
- | | | + | |[[Paris/July 27| July 27]] |
|} | |} | ||
Revision as of 11:41, 30 July 2007
Contents |
PCR products
PCR products | |||||
---|---|---|---|---|---|
Number | Name | Length | Comments | Product Left ? | Date |
P1 | Lox71-FtsA-FtsZ-1 | 1537 | Ø | ||
P2 | FtsZ-2 | 1088 | Ø | ||
P3 | DGAT-1 | ||||
P4 | DGAT-2 | ||||
P5 | Lox66-DapAColi | 985 | Ø | July 17 | |
P5' | Lox66-DapAColi | 985 | July 27 | ||
P6 | Lox66-DapASubtilis | 979 | Ø | July 17 | |
P6' | Lox66-DapASubtilis | 979 | July 27 | ||
P7 | Lox71-FtsZ | 1260 | EcoRI site in FtsZ not mutated | July 17 | |
P8 | RBS-DapAColi | 952 | B0030-DapAColi | Ø | July 24 |
P8' | RBS-DapAColi | 952 | B0030-DapAColi | July 27 | |
P9 | RBS-DapASubtilis | 946 | B0030-DapASubtilis | Ø | July 24 |
P9' | RBS-DapASubtilis | 946 | B0030-DapASubtilis | July 27 | |
P10 | Lox71-FtsA-FtsZ | 2580 | EcoRI site in FtsZ not mutated | July 24 | |
P11 | Lox71-KmR-Lox66 | 1084 | July 27 |
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products | ||||
---|---|---|---|---|
Number | Name | Description | Product finished ? | Date |
MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | July 15 | |
MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2) | MP2.2 | July 15 |
MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) | July 16 | |
MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | July 16 | |
MP5 | BBa_B0030 in pSB1A2 | Strong RBS (clones 1 + 2) | July 18 | |
MP6 | BBa_E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | July 18 | |
MP6' | BBa_E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | July 24 | |
MP7 | BBa_E0241 in pSB1A2 | PoPs to GFP converter (clones 1 + 2) | July 18 | |
MP7' | BBa_E0241 in pSB1A2 | PoPs to GFP converter (clones 1) | July 24 | |
MP8 | BBa_E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) | July 18 | |
MP9 | BBa_J61047 in pSB1A2 | Cre ORF (clones 1 + 2) | July 18 | |
MP9' | BBa_J61047 in pSB1A2 | Cre ORF (clones 1 + 2) | July 24 | |
MP10 | BBa_I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | July 20 | |
MP11 | BBa_pJ23107 in p61002 | Medium constitutive promoter in J61002 (clones 3 + 4) | MP11.3 | July 20 |
MP11' | BBa_pJ23107 in p61002 | Medium constitutive promoter in J61002 (clones 3) | July 23 |
Digestion Products
PURPLE = Vector
GREEN = Insert
Digestion Products | ||||||||
---|---|---|---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | Product Left | Date |
D1 | pJ23100 | pJ23100 | Spe1 | Pst1 | 2096 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | July 17 | |
D2 | mRFP | pJ23100 | Spe1 | Pst1 | 887 | July 17 | ||
D3 | pJ23107 | pJ23107 | Spe1 | Pst1 | 2096 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | July 17 | |
D4 | mRFP | pJ23107 | Spe1 | Pst1 | 887 | July 17 | ||
D5 | pJ23100 | pJ23100 | EcoR1 | Spe1 | 2925 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | July 17 | |
D6 | pJ23107 | pJ23107 | EcoR1 | Spe1 | 2925 | The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. | July 17 | |
D7 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | 977 | July 17 | ||
D8 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | 962 | July 17 | ||
D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 2925 | Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | July 21 | |
D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 58 | J23100 promoter ready for use as a fw-insert | July 21 | |
D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 2925 | Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | July 21 | |
D12 | pJ23107 promoter fw-insert | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 58 | J23107 promoter ready for usage as a fw-insert | July 21 | |
D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | 887 | mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it | July 21 | |
D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | 1063 | Cre ORF extracted for usage as a bw-insert | Ø D14.1 | July 21 |
D15 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | 2076 | bacward vector for cloning a CDS bw-insert after the B0030 RBS | July 21 | |
D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | ~960 | PCR product digested for cloning as a BioBrick | Ø | July 21 |
D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | ~960 | PCR product digested for cloning as a BioBrick | Ø | July 21 |
D20 | bw-insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | ~960 | PCR product digested for usage as a backward insert | Ø | July 21 |
D21 | bw-insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | ~960 | PCR product digested for usage as a backward insert | Ø | July 21 |
D22 | pSB1A2 open vector | BBa_J61047 (MP9.1 & MP9.2) | EcoI | PstI | Ø D22.1 | Open pSB1A2 vector for BioBrick EcoRI/PstI insertio | D22.1 Ø | July 23 |
D23 | AraC/pBad promoter fw-insert | BBa_I0500 (MP4.1 & MP4.2) | EcoI | SpeI | 1233 | AraC/pBad promoter ready for use as a forward insert | Ø D23.1 | July 23 |
D24 | eCFP bw-insert | BBa_E0422 (MP6.1 & MP6.2) | XbaI | PstI | eCFP (RBS-OFR_LVA-T) ready for use as a backward insert | July 23 | ||
D25 | PoPs to GFP converter | BBa_E0421 (MP7.1 & MP7.2) | XbaI | PstI | PoPs to GFP converter ready for use as a backward insert | July 23 | ||
D26 | gfp-tripart | BBa_E0840 (MP8.1 & MP8.2) | XbaI | PstI | gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert | July 23 | ||
D27 | lox71-ftsZ bw-insert | lox71-ftsZ PCR product P7 | XbaI | PstI | lox71-ftsZ(unmutated) ready for use as a backward insert | Ø | July 23 | |
D28 | RBS-DapAColi BB | P8 (RBS-DapAColi) | EcoRI | PstI | ~950 | Biobrick | July 25 | |
D29 | RBS-DapAColi | P8 (RBS-DapAColi) | XbaI | PstI | ~950 | Backward insert | July 25 | |
D30 | RBS-DapASubtilis BB | P9 (RBS-DapASubtilis) | EcoRI | PstI | ~950 | Biobrick | July 25 | |
D31 | RBS-DapASubtilis | P9 (RBS-DapASubtilis) | XbaI | PstI | ~950 | Backward insert | July 25 | |
D32 | Lox71-FtsA-FtsZ | P10 Lox71-FtsA-FtsZ | XbaI | PstI | ~2600 | Backward insert - EcoRI site still presents : we can't use EcoRI | July 25 |
Unexpected digestion results
Unexpected results | ||||||
---|---|---|---|---|---|---|
Number | Matrix Name | Enzyme 1 | Enzyme 2 | Expected Size | Description | Date |
D16 | pSB1AK3::B0015 (MP3.1 & MP3.2) | EcoR1 | Pst1 | ~3000 | We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3 | July 21 |
D17 | pSB1A2::E0422 (MP6.1 & MP6.2) | EcoRI | PstI | ~2040 + ~920 | 3 bands | July 21 |
Strains (glycerol stock)
Strains glycerol stock | ||||
---|---|---|---|---|
Number | Name | Description | Verified clone | Date |
S1 | MG1655 | Standard E.Coli strain | ||
S2 | Acinetobacter ADP1 | Strain used for TG coloration test with Red Nile | ||
S3 | DH5alpha pKs::DGAT | Enzyme catalysing the transormation of DAG into TG | ||
S4 | BBa_pJ23100 | Strong constitutive promoter in J61002 (clones 1 + 2) | ||
S5 | BBa_B0015 | Double terminator (clones 1 + 2) | ||
S6 | BBa_pJ23107 | Medium constitutive promoter in J61002 (clones 1 + 2) | July 19 | |
S7 | BBa_B0030 | Strong RBS (clones 1 + 2) | July 19 | |
S8 | BBa_E0422 | ECFP (RBS+LVA+Term) (clones 1 + 2) | July 19 | |
S9 | BBa_E0241 | PoPs to GFP converter (clones 1 + 2) | July 19 | |
S10 | BBa_E0840 | gfp tri-part; strong rbs (clones 1 + 2) | July 19 | |
S11 | BBa_J61047 | Cre ORF (clones 1 + 2) | July 19 | |
S12 | W121 | 05/07/07 | ||
S13 | BBa_I0500 | pBAD AraC (clone 1+2) | July 20 | |
S14 | BBa_pJ23107 | Medium constitutive promoter in J61002 (clone 3+4) | July 20 | |
S15 | BBa_P1003 | Kanamycin resistance cassette (clone 1+2) | July 20 | |
S16 | J61002::I0500 | AraC pBad regulating mRFP expression in pJ61002 (L1) | July 27 | |
S17 | pSB1A2::Lox66-DapA Coli | Lox66-DapA Coli cloned as a biobrick (L2) | July 27 | |
S18 | pSB1A2::B0030 + J61047 | RBS(B0030)-Cre ORF (L6) | July 27 | |
S19 | pSB1A2::BBa_J37026 | Lox66 (L9) | July 27 | |
S20 | pSB1A2::BBa_J37028 | Lox71 (L10) | July 27 |
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3