Paris/Freezer

From 2007.igem.org

(Difference between revisions)
Line 132: Line 132:
|Strong constitutive promoter in J61002 (clones 1 + 2)
|Strong constitutive promoter in J61002 (clones 1 + 2)
|
|
-
| 15/07/07
+
| [[Paris/July 15| July 15]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP2
| style="background: #ccffcc;" |MP2
Line 138: Line 138:
|Medium constitutive promoter in J61002 (clones 1 + 2)
|Medium constitutive promoter in J61002 (clones 1 + 2)
|MP2.2  
|MP2.2  
-
| 15/07/07
+
| [[Paris/July 15| July 15]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP3
| style="background: #ccffcc;" |MP3
Line 144: Line 144:
|Double terminator (clones 1 + 2)
|Double terminator (clones 1 + 2)
|
|
-
| 16/07/07
+
| [[Paris/July 16| July 16]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP4
| style="background: #ccffcc;" |MP4
Line 150: Line 150:
|AraC pBAD (clones 1 + 2)
|AraC pBAD (clones 1 + 2)
|
|
-
| 16/07/07
+
| [[Paris/July 16| July 16]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP5
| style="background: #ccffcc;" |MP5
Line 156: Line 156:
|Strong RBS (clones 1 + 2)
|Strong RBS (clones 1 + 2)
|
|
-
| 18/07/07
+
| [[Paris/July 18| July 18]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP6
| style="background: #ccffcc;" |MP6
Line 162: Line 162:
|ECFP (RBS+LVA+Term) (clones 1 + 2)
|ECFP (RBS+LVA+Term) (clones 1 + 2)
|
|
-
| 18/07/07
+
| [[Paris/July 18| July 18]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP6'
| style="background: #ccffcc;" |MP6'
Line 174: Line 174:
|PoPs to GFP converter (clones 1 + 2)
|PoPs to GFP converter (clones 1 + 2)
|
|
-
| 18/07/07
+
| [[Paris/July 18| July 18]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP7'
| style="background: #ccffcc;" |MP7'
Line 186: Line 186:
|gfp tri-part; strong rbs (clones 1 + 2)
|gfp tri-part; strong rbs (clones 1 + 2)
|
|
-
| 18/07/07
+
| [[Paris/July 18| July 18]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP9
| style="background: #ccffcc;" |MP9
Line 192: Line 192:
|Cre ORF (clones 1 + 2)
|Cre ORF (clones 1 + 2)
|
|
-
| 18/07/07
+
| [[Paris/July 18| July 18]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP9'
| style="background: #ccffcc;" |MP9'
Line 204: Line 204:
|AraC pBAD (clones 1 + 2)
|AraC pBAD (clones 1 + 2)
|
|
-
| 20/07/07
+
| [[Paris/July 20| July 20]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP11
| style="background: #ccffcc;" |MP11
Line 210: Line 210:
|Medium constitutive promoter in J61002 (clones 3 + 4)
|Medium constitutive promoter in J61002 (clones 3 + 4)
|MP11.3
|MP11.3
-
| 20/07/07
+
| [[Paris/July 20| July 20]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |MP11'
| style="background: #ccffcc;" |MP11'
Line 216: Line 216:
|Medium constitutive promoter in J61002 (clones 3)
|Medium constitutive promoter in J61002 (clones 3)
|
|
-
| 23/07/07
+
| [[Paris/July 23| July 23]]
|}
|}
Line 245: Line 245:
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert  
|Vector with the J23100 promoter ready for insertion of a RBS/CDS insert  
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D2
| style="background: #ccffcc;" |D2
Line 255: Line 255:
|
|
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D3
| style="background: #FF99FF;" |D3
Line 265: Line 265:
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert  
|Vector with the J23107 promoter ready for insertion of a RBS/CDS insert  
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D4
| style="background: #FF99FF;" |D4
Line 275: Line 275:
|
|
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D5
| style="background: #FF99FF;" |D5
Line 285: Line 285:
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D6
| style="background: #FF99FF;" |D6
Line 295: Line 295:
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D7
| style="background: #ccffcc;" |D7
Line 305: Line 305:
|
|
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D8
| style="background: #ccffcc;" |D8
Line 315: Line 315:
|
|
|
|
-
|17/07/07
+
|[[Paris/July 17| July 17]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D9
| style="background: #FF99FF;" |D9
Line 325: Line 325:
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D10
| style="background: #ccffcc;" |D10
Line 335: Line 335:
|J23100 promoter ready for use as a fw-insert
|J23100 promoter ready for use as a fw-insert
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D11
| style="background: #FF99FF;" |D11
Line 345: Line 345:
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D12
| style="background: #ccffcc;" |D12
Line 355: Line 355:
|J23107 promoter ready for usage as a fw-insert
|J23107 promoter ready for usage as a fw-insert
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D13
| style="background: #ccffcc;" |D13
Line 365: Line 365:
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D14
| style="background: #ccffcc;" |D14
Line 375: Line 375:
|Cre ORF extracted for usage as a bw-insert
|Cre ORF extracted for usage as a bw-insert
|Ø D14.1
|Ø D14.1
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D15
| style="background: #FF99FF;" |D15
Line 385: Line 385:
|bacward vector for cloning a CDS bw-insert after the B0030 RBS
|bacward vector for cloning a CDS bw-insert after the B0030 RBS
|
|
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D18
| style="background: #ccffcc;" |D18
Line 395: Line 395:
|PCR product digested for cloning as a BioBrick
|PCR product digested for cloning as a BioBrick
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D19
| style="background: #ccffcc;" |D19
Line 405: Line 405:
|PCR product digested for cloning as a BioBrick
|PCR product digested for cloning as a BioBrick
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D20
| style="background: #ccffcc;" |D20
Line 415: Line 415:
|PCR product digested for usage as a backward insert
|PCR product digested for usage as a backward insert
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D21
| style="background: #ccffcc;" |D21
Line 425: Line 425:
|PCR product digested for usage as a backward insert
|PCR product digested for usage as a backward insert
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #FF99FF;" |D22
| style="background: #FF99FF;" |D22
Line 435: Line 435:
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
|D22.1 Ø
|D22.1 Ø
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D23
| style="background: #ccffcc;" |D23
Line 445: Line 445:
|AraC/pBad promoter ready for use as a forward insert
|AraC/pBad promoter ready for use as a forward insert
|Ø D23.1
|Ø D23.1
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D24
| style="background: #ccffcc;" |D24
Line 455: Line 455:
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
|
|
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D25
| style="background: #ccffcc;" |D25
Line 465: Line 465:
|PoPs to GFP converter ready for use as a backward insert
|PoPs to GFP converter ready for use as a backward insert
|
|
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D26
| style="background: #ccffcc;" |D26
Line 475: Line 475:
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
|
|
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D27
| style="background: #ccffcc;" |D27
Line 485: Line 485:
|lox71-ftsZ(unmutated) ready for use as a backward insert
|lox71-ftsZ(unmutated) ready for use as a backward insert
-
|23/07/07
+
|[[Paris/July 23| July 23]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D28
| style="background: #ccffcc;" |D28
Line 495: Line 495:
|Biobrick
|Biobrick
|
|
-
|25/07/07
+
|[[Paris/July 25| July 25]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D29
| style="background: #ccffcc;" |D29
Line 505: Line 505:
|Backward insert
|Backward insert
|
|
-
|25/07/07
+
|[[Paris/July 25| July 25]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D30
| style="background: #ccffcc;" |D30
Line 515: Line 515:
|Biobrick
|Biobrick
|
|
-
|25/07/07
+
|[[Paris/July 25| July 25]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D31
| style="background: #ccffcc;" |D31
Line 525: Line 525:
|Backward insert
|Backward insert
|
|
-
|25/07/07
+
|[[Paris/July 25| July 25]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D32
| style="background: #ccffcc;" |D32
Line 535: Line 535:
|Backward insert - EcoRI site still presents : we can't use EcoRI
|Backward insert - EcoRI site still presents : we can't use EcoRI
|
|
-
|25/07/07
+
|[[Paris/July 25| July 25]]
|}
|}
Line 557: Line 557:
|~3000
|~3000
|We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3
|We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D17
| style="background: #ccffcc;" |D17
Line 565: Line 565:
|~2040 + ~920
|~2040 + ~920
|3 bands  
|3 bands  
-
|21/07/07
+
|[[Paris/July 21| July 21]]
|}
|}
Line 613: Line 613:
|Medium constitutive promoter in J61002 (clones 1 + 2)  
|Medium constitutive promoter in J61002 (clones 1 + 2)  
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S7
| style="background: #ccffcc;" |S7
Line 619: Line 619:
|Strong RBS (clones 1 + 2)
|Strong RBS (clones 1 + 2)
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S8
| style="background: #ccffcc;" |S8
Line 625: Line 625:
|ECFP (RBS+LVA+Term) (clones 1 + 2)  
|ECFP (RBS+LVA+Term) (clones 1 + 2)  
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S9
| style="background: #ccffcc;" |S9
Line 631: Line 631:
|PoPs to GFP converter (clones 1 + 2)
|PoPs to GFP converter (clones 1 + 2)
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S10
| style="background: #ccffcc;" |S10
Line 637: Line 637:
|gfp tri-part; strong rbs (clones 1 + 2)
|gfp tri-part; strong rbs (clones 1 + 2)
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S11
| style="background: #ccffcc;" |S11
Line 643: Line 643:
|Cre ORF (clones 1 + 2)
|Cre ORF (clones 1 + 2)
|
|
-
|19/07/07
+
|[[Paris/July 19| July 19]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S12
| style="background: #ccffcc;" |S12
Line 655: Line 655:
|pBAD AraC (clone 1+2)
|pBAD AraC (clone 1+2)
|
|
-
|20/07/07
+
|[[Paris/July 20| July 20]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S14
| style="background: #ccffcc;" |S14
Line 661: Line 661:
|Medium constitutive promoter in J61002 (clone 3+4)
|Medium constitutive promoter in J61002 (clone 3+4)
|
|
-
|20/07/07
+
|[[Paris/July 20| July 20]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S15
| style="background: #ccffcc;" |S15
Line 667: Line 667:
|Kanamycin resistance cassette (clone 1+2)
|Kanamycin resistance cassette (clone 1+2)
|
|
-
|20/07/07
+
|[[Paris/July 20| July 20]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S16
| style="background: #ccffcc;" |S16
Line 673: Line 673:
|AraC pBad regulating mRFP expression in pJ61002 (L1)
|AraC pBad regulating mRFP expression in pJ61002 (L1)
|
|
-
|27/07/07
+
|[[Paris/July 27| July 27]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S17
| style="background: #ccffcc;" |S17
Line 679: Line 679:
|Lox66-DapA Coli cloned as a biobrick (L2)
|Lox66-DapA Coli cloned as a biobrick (L2)
|
|
-
|27/07/07
+
|[[Paris/July 27| July 27]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S18
| style="background: #ccffcc;" |S18
Line 685: Line 685:
|RBS(B0030)-Cre ORF (L6)
|RBS(B0030)-Cre ORF (L6)
|
|
-
|27/07/07
+
|[[Paris/July 27| July 27]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S19
| style="background: #ccffcc;" |S19
Line 691: Line 691:
|Lox66 (L9)
|Lox66 (L9)
|
|
-
|27/07/07
+
|[[Paris/July 27| July 27]]
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |S20
| style="background: #ccffcc;" |S20
Line 697: Line 697:
|Lox71 (L10)
|Lox71 (L10)
|
|
-
|27/07/07
+
|[[Paris/July 27| July 27]]
|}
|}

Revision as of 11:41, 30 July 2007

Contents

PCR products

PCR products
Number Name Length Comments Product Left ? Date
P1 Lox71-FtsA-FtsZ-1 1537 Ø
P2 FtsZ-2 1088 Ø
P3 DGAT-1
P4 DGAT-2
P5 Lox66-DapAColi 985 Ø July 17
P5' Lox66-DapAColi 985 July 27
P6 Lox66-DapASubtilis 979 Ø July 17
P6' Lox66-DapASubtilis 979 July 27
P7 Lox71-FtsZ 1260 EcoRI site in FtsZ not mutated July 17
P8 RBS-DapAColi 952 B0030-DapAColi Ø July 24
P8' RBS-DapAColi 952 B0030-DapAColi July 27
P9 RBS-DapASubtilis 946 B0030-DapASubtilis Ø July 24
P9' RBS-DapASubtilis 946 B0030-DapASubtilis July 27
P10 Lox71-FtsA-FtsZ 2580 EcoRI site in FtsZ not mutated July 24
P11 Lox71-KmR-Lox66 1084 July 27

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Product finished ? Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2) July 15
MP2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2) MP2.2 July 15
MP3 B0015 in pSB1AK3 Double terminator (clones 1 + 2) July 16
MP4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2) July 16
MP5 BBa_B0030 in pSB1A2 Strong RBS (clones 1 + 2) July 18
MP6 BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) July 18
MP6' BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) July 24
MP7 BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2) July 18
MP7' BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1) July 24
MP8 BBa_E0840 in pSB1A2 gfp tri-part; strong rbs (clones 1 + 2) July 18
MP9 BBa_J61047 in pSB1A2 Cre ORF (clones 1 + 2) July 18
MP9' BBa_J61047 in pSB1A2 Cre ORF (clones 1 + 2) July 24
MP10 BBa_I0500 in pSB2K3 AraC pBAD (clones 1 + 2) July 20
MP11 BBa_pJ23107 in p61002 Medium constitutive promoter in J61002 (clones 3 + 4) MP11.3 July 20
MP11' BBa_pJ23107 in p61002 Medium constitutive promoter in J61002 (clones 3) July 23

Digestion Products


PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left Date
D1 pJ23100 pJ23100 Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert July 17
D2 mRFP pJ23100 Spe1 Pst1 887 July 17
D3 pJ23107 pJ23107 Spe1 Pst1 2096 Vector with the J23107 promoter ready for insertion of a RBS/CDS insert July 17
D4 mRFP pJ23107 Spe1 Pst1 887 July 17
D5 pJ23100 pJ23100 EcoR1 Spe1 2925 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. July 17
D6 pJ23107 pJ23107 EcoR1 Spe1 2925 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. July 17
D7 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) EcoR1 Pst1 977 July 17
D8 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) Xba1 Pst1 962 July 17
D9 pJ23100 ES vector pJ23100 (MP1.1 & MP1.2) EcoI SpeI 2925 Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) July 21
D10 pJ23100 promoter fw-insert pJ23100 (MP1.1 & MP1.2) EcoI SpeI 58 J23100 promoter ready for use as a fw-insert July 21
D11 pJ23107 ES vector pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 2925 Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter July 21
D12 pJ23107 promoter fw-insert pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 58 J23107 promoter ready for usage as a fw-insert July 21
D13 mRFP pJ23107 (MP2.2, MP2.3 & MP2.4) SpeI PstI 887 mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to add any biobrick after it July 21
D14 Cre ORF J61047 MP9.1 & MP9.2 XbaI PstI 1063 Cre ORF extracted for usage as a bw-insert Ø D14.1 July 21
D15 B0030 bw-vector B0030 MP5.1 & MP5.2 SpeI PstI 2076 bacward vector for cloning a CDS bw-insert after the B0030 RBS July 21
D18 BB dig. lox66DapAE.coli lox66-DapAE.coli P5 PCR product EcoRI PstI ~960 PCR product digested for cloning as a BioBrick Ø July 21
D19 BB dig. lox66DapAsubtilis lox66-DapAsubtilis P6 PCR product EcoRI PstI ~960 PCR product digested for cloning as a BioBrick Ø July 21
D20 bw-insert lox66-DapAE.coli lox66-DapAE.coli P5 PCR product XbaI PstI ~960 PCR product digested for usage as a backward insert Ø July 21
D21 bw-insert lox66-DapAsubtilis lox66-DapAsubtilis P6 PCR product XbaI PstI ~960 PCR product digested for usage as a backward insert Ø July 21
D22 pSB1A2 open vector BBa_J61047 (MP9.1 & MP9.2) EcoI PstI Ø D22.1 Open pSB1A2 vector for BioBrick EcoRI/PstI insertio D22.1 Ø July 23
D23 AraC/pBad promoter fw-insert BBa_I0500 (MP4.1 & MP4.2) EcoI SpeI 1233 AraC/pBad promoter ready for use as a forward insert Ø D23.1 July 23
D24 eCFP bw-insert BBa_E0422 (MP6.1 & MP6.2) XbaI PstI eCFP (RBS-OFR_LVA-T) ready for use as a backward insert July 23
D25 PoPs to GFP converter BBa_E0421 (MP7.1 & MP7.2) XbaI PstI PoPs to GFP converter ready for use as a backward insert July 23
D26 gfp-tripart BBa_E0840 (MP8.1 & MP8.2) XbaI PstI gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert July 23
D27 lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert Ø July 23
D28 RBS-DapAColi BB P8 (RBS-DapAColi) EcoRI PstI ~950 Biobrick July 25
D29 RBS-DapAColi P8 (RBS-DapAColi) XbaI PstI ~950 Backward insert July 25
D30 RBS-DapASubtilis BB P9 (RBS-DapASubtilis) EcoRI PstI ~950 Biobrick July 25
D31 RBS-DapASubtilis P9 (RBS-DapASubtilis) XbaI PstI ~950 Backward insert July 25
D32 Lox71-FtsA-FtsZ P10 Lox71-FtsA-FtsZ XbaI PstI ~2600 Backward insert - EcoRI site still presents : we can't use EcoRI July 25

Unexpected digestion results

Unexpected results
Number Matrix Name Enzyme 1 Enzyme 2 Expected Size Description Date
D16 pSB1AK3::B0015 (MP3.1 & MP3.2) EcoR1 Pst1 ~3000 We have one band at ~2000. Furthermore, the strain which is suppose to be resistant to Kanamycin is not. We suspect that the part is in the pSB1A2 instead of the pSB1AK3 July 21
D17 pSB1A2::E0422 (MP6.1 & MP6.2) EcoRI PstI ~2040 + ~920 3 bands July 21

Strains (glycerol stock)

Strains glycerol stock
Number Name Description Verified clone Date
S1 MG1655 Standard E.Coli strain
S2 Acinetobacter ADP1 Strain used for TG coloration test with Red Nile
S3 DH5alpha pKs::DGAT Enzyme catalysing the transormation of DAG into TG
S4 BBa_pJ23100 Strong constitutive promoter in J61002 (clones 1 + 2)
S5 BBa_B0015 Double terminator (clones 1 + 2)
S6 BBa_pJ23107 Medium constitutive promoter in J61002 (clones 1 + 2) July 19
S7 BBa_B0030 Strong RBS (clones 1 + 2) July 19
S8 BBa_E0422 ECFP (RBS+LVA+Term) (clones 1 + 2) July 19
S9 BBa_E0241 PoPs to GFP converter (clones 1 + 2) July 19
S10 BBa_E0840 gfp tri-part; strong rbs (clones 1 + 2) July 19
S11 BBa_J61047 Cre ORF (clones 1 + 2) July 19
S12 W121 05/07/07
S13 BBa_I0500 pBAD AraC (clone 1+2) July 20
S14 BBa_pJ23107 Medium constitutive promoter in J61002 (clone 3+4) July 20
S15 BBa_P1003 Kanamycin resistance cassette (clone 1+2) July 20
S16 J61002::I0500 AraC pBad regulating mRFP expression in pJ61002 (L1) July 27
S17 pSB1A2::Lox66-DapA Coli Lox66-DapA Coli cloned as a biobrick (L2) July 27
S18 pSB1A2::B0030 + J61047 RBS(B0030)-Cre ORF (L6) July 27
S19 pSB1A2::BBa_J37026 Lox66 (L9) July 27
S20 pSB1A2::BBa_J37028 Lox71 (L10) July 27


  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3