Paris/Freezer

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(PCR products)
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== PCR products ==
== PCR products ==
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | PCR products
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=5%| Name
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|width=5%| Length
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|width=85%| Comments
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |1
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|Lox71-FtsA-FtsZ-1
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |2
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|FtsZ-2
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |3
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|DGAT-1
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |4
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|DGAT-2
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |5
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|Lox66-DapAColi
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |6
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|Lox66-DapASubtilis
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|
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|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |7
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|Lox71-FtsZ
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|
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|EcoR1 site not mutated
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* Lox71-FtsA-FtsZ-1
 
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* FtsZ-2
 
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* DGAT-1
 
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* DGAT-2
 
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* Lox66-DapAColi
 
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* Lox71-FtsZ (EcoR1 site not mutated)
 
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* Lox66-DapASubtilis
 
== Plasmids: MiniPrep Products ==
== Plasmids: MiniPrep Products ==

Revision as of 15:21, 18 July 2007

PCR products

PCR products
Number Name Length Comments
1 Lox71-FtsA-FtsZ-1
2 FtsZ-2
3 DGAT-1
4 DGAT-2
5 Lox66-DapAColi
6 Lox66-DapASubtilis
7 Lox71-FtsZ EcoR1 site not mutated


Plasmids: MiniPrep Products

  • pJ23100 : Strong constitutive promoter in J61002 (clones 1 + 2)
  • pJ23107 : Medium constitutive promoter in J61002 (clones 1 + 2)
  • B0015 : Double terminator in pSB1AK3 (clones 1 + 2)
  • I0500 : AraC pBAD in pSB2K3 (clones 1 + 2)

Strains (glycerol stock)

  • MG1655
  • Acinetobacter ADP1
  • w121
  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
  • DH5alpha pKs::DGAT
  • pJ23100 Clone1
  • pJ23100 Clone2
  • BBa_B0015 Clone1
  • BBa_B0015 Clone2