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Revision as of 15:21, 18 July 2007 (view source) David.bikard (Talk | contribs) ← Older edit		Revision as of 15:24, 18 July 2007 (view source) David.bikard (Talk | contribs) (→PCR products) Newer edit →
Line 11:		Line 11:
	| style="background: #ccffcc;" |1		| style="background: #ccffcc;" |1
	|Lox71-FtsA-FtsZ-1		|Lox71-FtsA-FtsZ-1
-	|	+	|1537
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |2		| style="background: #ccffcc;" |2
	|FtsZ-2		|FtsZ-2
-	|	+	|1088
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
Line 31:		Line 31:
	| style="background: #ccffcc;" |5		| style="background: #ccffcc;" |5
	|Lox66-DapAColi		|Lox66-DapAColi
-	|	+	|985
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |6		| style="background: #ccffcc;" |6
	|Lox66-DapASubtilis		|Lox66-DapASubtilis
-	|	+	|979
	|		|
	|- style="background: #cccccc;"		|- style="background: #cccccc;"
	| style="background: #ccffcc;" |7		| style="background: #ccffcc;" |7
	|Lox71-FtsZ		|Lox71-FtsZ
-	|	+	|1260
	|EcoR1 site not mutated		|EcoR1 site not mutated
	|}		|}

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Revision as of 15:24, 18 July 2007

PCR products

PCR products
Number	Name	Length	Comments
1	Lox71-FtsA-FtsZ-1	1537
2	FtsZ-2	1088
3	DGAT-1
4	DGAT-2
5	Lox66-DapAColi	985
6	Lox66-DapASubtilis	979
7	Lox71-FtsZ	1260	EcoR1 site not mutated

Plasmids: MiniPrep Products

• pJ23100 : Strong constitutive promoter in J61002 (clones 1 + 2)
• pJ23107 : Medium constitutive promoter in J61002 (clones 1 + 2)
• B0015 : Double terminator in pSB1AK3 (clones 1 + 2)
• I0500 : AraC pBAD in pSB2K3 (clones 1 + 2)

Strains (glycerol stock)

• MG1655
• Acinetobacter ADP1
• w121
• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3
• DH5alpha pKs::DGAT
• pJ23100 Clone1
• pJ23100 Clone2
• BBa_B0015 Clone1
• BBa_B0015 Clone2