Paris/Freezer

From 2007.igem.org

(Difference between revisions)
(PCR products)
(Plasmids: MiniPrep Products)
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== Plasmids: MiniPrep Products ==
== Plasmids: MiniPrep Products ==
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+
{|
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* pJ23100 : Strong constitutive promoter in J61002 (clones 1 + 2)
+
|- style="background: #ccccff;"
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* pJ23107 : Medium constitutive promoter in J61002 (clones 1 + 2)
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! colspan="5" style="background: #ccccff;" | PCR products
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* B0015 : Double terminator in pSB1AK3 (clones 1 + 2)
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|- style="background: #ccccff; text-align: center;"
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* I0500 : AraC pBAD in pSB2K3 (clones 1 + 2)
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|width=5%| Number
 +
|width=5%| Name
 +
|width=5%| Description
 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |1
 +
|<bbpart>pJ23100</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |2
 +
|<bbpart>pJ23107</bbpart>
 +
|Medium constitutive promoter in J61002 (clones 1 + 2)
 +
|
 +
|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |3
 +
|<bbpart>B0015</bbpart>
 +
|Double terminator in pSB1AK3 (clones 1 + 2)
 +
|
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" |3
 +
|<bbpart>I0500</bbpart>
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|AraC pBAD in pSB2K3 (clones 1 + 2)
 +
|}
== Strains (glycerol stock) ==
== Strains (glycerol stock) ==

Revision as of 15:29, 18 July 2007

PCR products

PCR products
Number Name Length Comments
1 Lox71-FtsA-FtsZ-1 1537
2 FtsZ-2 1088
3 DGAT-1
4 DGAT-2
5 Lox66-DapAColi 985
6 Lox66-DapASubtilis 979
7 Lox71-FtsZ 1260 EcoR1 site not mutated

Plasmids: MiniPrep Products

PCR products
Number Name Description
1 pJ23100 Strong constitutive promoter in J61002 (clones 1 + 2)
2 pJ23107 Medium constitutive promoter in J61002 (clones 1 + 2)
3 B0015 Double terminator in pSB1AK3 (clones 1 + 2)
3 I0500 AraC pBAD in pSB2K3 (clones 1 + 2)

Strains (glycerol stock)

  • MG1655
  • Acinetobacter ADP1
  • w121
  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
  • DH5alpha pKs::DGAT
  • pJ23100 Clone1
  • pJ23100 Clone2
  • BBa_B0015 Clone1
  • BBa_B0015 Clone2