Paris/Freezer
From 2007.igem.org
(Difference between revisions)
David.bikard (Talk | contribs) (→Plasmids: MiniPrep Products) |
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|<bbpart>pJ23100</bbpart> | |<bbpart>pJ23100</bbpart> | ||
|Strong constitutive promoter in J61002 (clones 1 + 2) | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
- | |||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |2 | | style="background: #ccffcc;" |2 | ||
|<bbpart>pJ23107</bbpart> | |<bbpart>pJ23107</bbpart> | ||
|Medium constitutive promoter in J61002 (clones 1 + 2) | |Medium constitutive promoter in J61002 (clones 1 + 2) | ||
- | |||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |3 | | style="background: #ccffcc;" |3 | ||
|<bbpart>B0015</bbpart> | |<bbpart>B0015</bbpart> | ||
|Double terminator in pSB1AK3 (clones 1 + 2) | |Double terminator in pSB1AK3 (clones 1 + 2) | ||
- | |||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |3 | | style="background: #ccffcc;" |3 |
Revision as of 15:31, 18 July 2007
PCR products
PCR products | ||||
---|---|---|---|---|
Number | Name | Length | Comments | |
1 | Lox71-FtsA-FtsZ-1 | 1537 | ||
2 | FtsZ-2 | 1088 | ||
3 | DGAT-1 | |||
4 | DGAT-2 | |||
5 | Lox66-DapAColi | 985 | ||
6 | Lox66-DapASubtilis | 979 | ||
7 | Lox71-FtsZ | 1260 | EcoR1 site not mutated |
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products | ||||
---|---|---|---|---|
Number | Name | Description | ||
1 | pJ23100 | Strong constitutive promoter in J61002 (clones 1 + 2) | ||
2 | pJ23107 | Medium constitutive promoter in J61002 (clones 1 + 2) | ||
3 | B0015 | Double terminator in pSB1AK3 (clones 1 + 2) | ||
3 | I0500 | AraC pBAD in pSB2K3 (clones 1 + 2) |
Strains (glycerol stock)
- MG1655
- Acinetobacter ADP1
- w121
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
- DH5alpha pKs::DGAT
- pJ23100 Clone1
- pJ23100 Clone2
- BBa_B0015 Clone1
- BBa_B0015 Clone2