Paris/Freezer
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|width=5%| Date | |width=5%| Date | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP1 |
|<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | |<bbpart>pJ23100</bbpart> in <bbpart>J61002</bbpart> | ||
|Strong constitutive promoter in J61002 (clones 1 + 2) | |Strong constitutive promoter in J61002 (clones 1 + 2) | ||
| Line 78: | Line 78: | ||
| 15/07/07 | | 15/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP2 |
|<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart> | |<bbpart>pJ23107</bbpart> in <bbpart>J61002</bbpart> | ||
|Medium constitutive promoter in J61002 (clones 1 + 2) | |Medium constitutive promoter in J61002 (clones 1 + 2) | ||
| Line 84: | Line 84: | ||
| 15/07/07 | | 15/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP3 |
|<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> | |<bbpart>B0015</bbpart> in <bbpart>pSB1AK3</bbpart> | ||
|Double terminator (clones 1 + 2) | |Double terminator (clones 1 + 2) | ||
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| 16/07/07 | | 16/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP4 |
|<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart> | |<bbpart>I0500</bbpart> in <bbpart>pSB2K3</bbpart> | ||
|AraC pBAD (clones 1 + 2) | |AraC pBAD (clones 1 + 2) | ||
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| 16/07/07 | | 16/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP5 |
|<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart> | |<bbpart>BBa_B0030</bbpart> in <bbpart>pSB1A2</bbpart> | ||
|Strong RBS (clones 1 + 2) | |Strong RBS (clones 1 + 2) | ||
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| 18/07/07 | | 18/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP6 |
|<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart> | |<bbpart>BBa_E0422</bbpart> in <bbpart>pSB1A2</bbpart> | ||
|ECFP (RBS+LVA+Term) (clones 1 + 2) | |ECFP (RBS+LVA+Term) (clones 1 + 2) | ||
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| 18/07/07 | | 18/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP7 |
|<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart> | |<bbpart>BBa_E0241</bbpart> in <bbpart>pSB1A2</bbpart> | ||
|PoPs to GFP converter (clones 1 + 2) | |PoPs to GFP converter (clones 1 + 2) | ||
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| 18/07/07 | | 18/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP8 |
|<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> | |<bbpart>BBa_E0840</bbpart> in <bbpart>pSB1A2</bbpart> | ||
|gfp tri-part; strong rbs (clones 1 + 2) | |gfp tri-part; strong rbs (clones 1 + 2) | ||
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| 18/07/07 | | 18/07/07 | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| - | | style="background: #ccffcc;" | | + | | style="background: #ccffcc;" |MP9 |
|<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart> | |<bbpart>BBa_J61047</bbpart> in <bbpart>pSB1A2</bbpart> | ||
|Cre ORF (clones 1 + 2) | |Cre ORF (clones 1 + 2) | ||
Revision as of 18:18, 18 July 2007
Contents |
PCR products
| PCR products | |||||
|---|---|---|---|---|---|
| Number | Name | Length | Comments | Product Left ? | Date |
| 1 | Lox71-FtsA-FtsZ-1 | 1537 | |||
| 2 | FtsZ-2 | 1088 | |||
| 3 | DGAT-1 | ||||
| 4 | DGAT-2 | ||||
| 5 | Lox66-DapAColi | 985 | |||
| 6 | Lox66-DapASubtilis | 979 | 17/07/07 | ||
| 7 | Lox71-FtsZ | 1260 | EcoR1 site not mutated | 17/07/07 | |
Plasmids: MiniPrep Products
| Plasmids: MiniPrep Products | ||||
|---|---|---|---|---|
| Number | Name | Description | Product Left ? | Date |
| MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | 15/07/07 | |
| MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2) | 15/07/07 | |
| MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) | 16/07/07 | |
| MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | 16/07/07 | |
| MP5 | BBa_B0030 in pSB1A2 | Strong RBS (clones 1 + 2) | 18/07/07 | |
| MP6 | BBa_E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | 18/07/07 | |
| MP7 | BBa_E0241 in pSB1A2 | PoPs to GFP converter (clones 1 + 2) | 18/07/07 | |
| MP8 | BBa_E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) | 18/07/07 | |
| MP9 | BBa_J61047 in pSB1A2 | Cre ORF (clones 1 + 2) | 18/07/07 | |
Digestion Products
| Digestion Products | |||||||
|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Description | Product Left | Date |
| D1 | pJ23100 | EcoR1 | Spe1 | ||||
| D2 | pJ23100 | Spe1 | Pst1 | ||||
| D3 | pJ23107 | EcoR1 | Spe1 | ||||
| D4 | pJ23107 | Spe1 | Pst1 | ||||
| D5 | pSB2K3 | EcoR1 | Pst1 | ||||
| D6 | pSB1AK3 | EcoR1 | Pst1 | ||||
| D7 | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | ||||
| D8 | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | ||||
Strains (glycerol stock)
- MG1655
- Acinetobacter ADP1
- w121
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
- DH5alpha pKs::DGAT
- pJ23100 Clone1
- pJ23100 Clone2
- BBa_B0015 Clone1
- BBa_B0015 Clone2
