Paris/Freezer
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David.bikard (Talk | contribs) (→Digestion Products) |
David.bikard (Talk | contribs) (→Digestion Products) |
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|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D7 | | style="background: #ccffcc;" |D7 | ||
+ | |Lox66 DapA E.coli | ||
|Lox66 DapA E.coli (PCR product) | |Lox66 DapA E.coli (PCR product) | ||
- | |||
|EcoR1 | |EcoR1 | ||
|Pst1 | |Pst1 | ||
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|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D8 | | style="background: #ccffcc;" |D8 | ||
+ | |Lox66 DapA E.coli | ||
|Lox66 DapA E.coli (PCR product) | |Lox66 DapA E.coli (PCR product) | ||
- | |||
|Xba1 | |Xba1 | ||
|Pst1 | |Pst1 |
Revision as of 11:40, 19 July 2007
Contents |
PCR products
PCR products | |||||
---|---|---|---|---|---|
Number | Name | Length | Comments | Product Left ? | Date |
P1 | Lox71-FtsA-FtsZ-1 | 1537 | |||
P2 | FtsZ-2 | 1088 | |||
P3 | DGAT-1 | ||||
P4 | DGAT-2 | ||||
P5 | Lox66-DapAColi | 985 | |||
P6 | Lox66-DapASubtilis | 979 | 17/07/07 | ||
P7 | Lox71-FtsZ | 1260 | EcoR1 site not mutated | 17/07/07 |
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products | ||||
---|---|---|---|---|
Number | Name | Description | Product Left ? | Date |
MP1 | pJ23100 in J61002 | Strong constitutive promoter in J61002 (clones 1 + 2) | 15/07/07 | |
MP2 | pJ23107 in J61002 | Medium constitutive promoter in J61002 (clones 1 + 2) | 15/07/07 | |
MP3 | B0015 in pSB1AK3 | Double terminator (clones 1 + 2) | 16/07/07 | |
MP4 | I0500 in pSB2K3 | AraC pBAD (clones 1 + 2) | 16/07/07 | |
MP5 | BBa_B0030 in pSB1A2 | Strong RBS (clones 1 + 2) | 18/07/07 | |
MP6 | BBa_E0422 in pSB1A2 | ECFP (RBS+LVA+Term) (clones 1 + 2) | 18/07/07 | |
MP7 | BBa_E0241 in pSB1A2 | PoPs to GFP converter (clones 1 + 2) | 18/07/07 | |
MP8 | BBa_E0840 in pSB1A2 | gfp tri-part; strong rbs (clones 1 + 2) | 18/07/07 | |
MP9 | BBa_J61047 in pSB1A2 | Cre ORF (clones 1 + 2) | 18/07/07 |
Digestion Products
Digestion Products | |||||||
---|---|---|---|---|---|---|---|
Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Description | Product Left | Date |
D1 | J61002 | pJ23100 | EcoR1 | Spe1 | The vector with the mRFP gene ready for insertion of a promoter | 17/07/07 | |
D2 | pJ23100 | pJ23100 | Spe1 | Pst1 | Vector with the J23100 promoter ready for insertion of a RBS/CDS insert | 17/07/07 | |
D3 | J61002 | pJ23107 | EcoR1 | Spe1 | The vector with the mRFP gene ready for insertion of a promoter | 17/07/07 | |
D4 | pJ23107 | pJ23107 | Spe1 | Pst1 | Vector with the J23107 promoter ready for insertion of a RBS/CDS insert | 17/07/07 | |
D5 | pSB2K3 | I0500 | EcoR1 | Pst1 | I'm not sure the digestion worked properly... this may not be the good fragment | 17/07/07 | |
D6 | pSB1AK3 | EcoR1 | Pst1 | 17/07/07 | |||
D7 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | EcoR1 | Pst1 | 17/07/07 | ||
D8 | Lox66 DapA E.coli | Lox66 DapA E.coli (PCR product) | Xba1 | Pst1 | 17/07/07 |
Strains (glycerol stock)
- MG1655
- Acinetobacter ADP1
- w121
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3
- DH5alpha pKs::DGAT
- pJ23100 Clone1
- pJ23100 Clone2
- BBa_B0015 Clone1
- BBa_B0015 Clone2