From 2007.igem.org

Revision as of 12:40, 19 July 2007 (view source) David.bikard (Talk | contribs) (→Strains (glycerol stock)) ← Older edit		Revision as of 12:46, 19 July 2007 (view source) David.bikard (Talk | contribs) (→Strains (glycerol stock)) Newer edit →
Line 248:		Line 248:
	|		|
	|		|
		+	|- style="background: #cccccc;"
		+	| style="background: #ccffcc;" |S6
		+	|pJ23107
		+	|
		+	|19/07/07
	|}		|}
-	pJ23107	+	|- style="background: #cccccc;"
-	I0500	+	| style="background: #ccffcc;" |S7
-	BBa_B0030	+	|BBa_B0030
		+
		+	|- style="background: #cccccc;"
		+	| style="background: #ccffcc;" |S7
	BBa_E0422		BBa_E0422
		+
		+	|- style="background: #cccccc;"
		+	| style="background: #ccffcc;" |S7
	BBa_E0241		BBa_E0241
		+
		+	|- style="background: #cccccc;"
		+	| style="background: #ccffcc;" |S7
	BBa_E0840		BBa_E0840
		+
		+	|- style="background: #cccccc;"
		+	| style="background: #ccffcc;" |S7
		+	BBa_J61047
		+
		+	I0500
		+
		+
		+
	BBa_J61047		BBa_J61047
	* MG1655		* MG1655

---

Revision as of 12:46, 19 July 2007

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock)

PCR products

PCR products
Number	Name	Length	Comments	Product Left ?	Date
P1	Lox71-FtsA-FtsZ-1	1537
P2	FtsZ-2	1088
P3	DGAT-1
P4	DGAT-2
P5	Lox66-DapAColi	985
P6	Lox66-DapASubtilis	979			17/07/07
P7	Lox71-FtsZ	1260	EcoR1 site not mutated		17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Product Left ?	Date
MP1	pJ23100 in J61002	Strong constitutive promoter in J61002 (clones 1 + 2)		15/07/07
MP2	pJ23107 in J61002	Medium constitutive promoter in J61002 (clones 1 + 2)		15/07/07
MP3	B0015 in pSB1AK3	Double terminator (clones 1 + 2)		16/07/07
MP4	I0500 in pSB2K3	AraC pBAD (clones 1 + 2)		16/07/07
MP5	BBa_B0030 in pSB1A2	Strong RBS (clones 1 + 2)		18/07/07
MP6	BBa_E0422 in pSB1A2	ECFP (RBS+LVA+Term) (clones 1 + 2)		18/07/07
MP7	BBa_E0241 in pSB1A2	PoPs to GFP converter (clones 1 + 2)		18/07/07
MP8	BBa_E0840 in pSB1A2	gfp tri-part; strong rbs (clones 1 + 2)		18/07/07
MP9	BBa_J61047 in pSB1A2	Cre ORF (clones 1 + 2)		18/07/07

Digestion Products

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Description	Product Left	Date
D1	J61002	pJ23100	EcoR1	Spe1	The vector with the mRFP gene ready for insertion of a promoter		17/07/07
D2	pJ23100	pJ23100	Spe1	Pst1	Vector with the J23100 promoter ready for insertion of a RBS/CDS insert		17/07/07
D3	J61002	pJ23107	EcoR1	Spe1	The vector with the mRFP gene ready for insertion of a promoter		17/07/07
D4	pJ23107	pJ23107	Spe1	Pst1	Vector with the J23107 promoter ready for insertion of a RBS/CDS insert		17/07/07
D5	pSB2K3	I0500	EcoR1	Pst1	I'm not sure the digestion worked properly... this may not be the good fragment		17/07/07
D6	pSB1AK3		EcoR1	Pst1			17/07/07
D7	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	EcoR1	Pst1			17/07/07
D8	Lox66 DapA E.coli	Lox66 DapA E.coli (PCR product)	Xba1	Pst1			17/07/07

Strains (glycerol stock)

Strains glycerol stock
Number	Name	Description	Date
S1	MG1655
S2	Acinetobacter ADP1
S3	DH5alpha pKs::DGAT
S4	pJ23100
S5	BBa_B0015
S6	pJ23107		19/07/07

|- style="background: #cccccc;" | style="background: #ccffcc;" |S7 |BBa_B0030

|- style="background: #cccccc;" | style="background: #ccffcc;" |S7 BBa_E0422

|- style="background: #cccccc;" | style="background: #ccffcc;" |S7 BBa_E0241

|- style="background: #cccccc;" | style="background: #ccffcc;" |S7 BBa_E0840

|- style="background: #cccccc;" | style="background: #ccffcc;" |S7 BBa_J61047

I0500

BBa_J61047

• MG1655
• Acinetobacter ADP1
• w121
• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3
• DH5alpha pKs::DGAT
• pJ23100 Clone1
• pJ23100 Clone2
• BBa_B0015 Clone1
• BBa_B0015 Clone2