Paris/Freezer

From 2007.igem.org

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(Digestion Products)
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|Lox66 DapA E.coli
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|Lox66 DapA E.coli (PCR product)

Revision as of 15:27, 19 July 2007

Contents

PCR products

PCR products
Number Name Length Comments Product Left ? Date
P1 Lox71-FtsA-FtsZ-1 1537
P2 FtsZ-2 1088
P3 DGAT-1
P4 DGAT-2
P5 Lox66-DapAColi 985
P6 Lox66-DapASubtilis 979 17/07/07
P7 Lox71-FtsZ 1260 EcoR1 site not mutated 17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Product Left ? Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP3 B0015 in pSB1AK3 Double terminator (clones 1 + 2) 16/07/07
MP4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2) 16/07/07
MP5 BBa_B0030 in pSB1A2 Strong RBS (clones 1 + 2) 18/07/07
MP6 BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) 18/07/07
MP7 BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2) 18/07/07
MP8 BBa_E0840 in pSB1A2 gfp tri-part; strong rbs (clones 1 + 2) 18/07/07
MP9 BBa_J61047 in pSB1A2 Cre ORF (clones 1 + 2) 18/07/07

Digestion Products

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Description Product Left Date
D1 J23100 pJ23100 Spe1 Pst1 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert 17/07/07
D2 mRFP pJ23100 Spe1 Pst1 17/07/07
D3 J23107 pJ23107 Spe1 Pst1 Vector with the J23107 promoter ready for insertion of a RBS/CDS insert 17/07/07
D4 mRFP pJ23107 Spe1 Pst1 17/07/07
D5 pJ23100 pJ23100 EcoR1 Spe1 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 17/07/07
D6 pJ23107 pJ23107 EcoR1 Spe1 The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly. 17/07/07
D7 pSB2K3 pSB2K3::I0500 EcoR1 Pst1 I'm not sure the digestion worked properly... this may not be the good fragment 17/07/07
D8 pSB1AK3 pSB1AK3::BBa_B0015 EcoR1 Pst1 17/07/07
D9 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) EcoR1 Pst1 17/07/07
D10 Lox66 DapA E.coli Lox66 DapA E.coli (PCR product) Xba1 Pst1 17/07/07

The vector with the mRFP gene ready for insertion of a promoter

Strains (glycerol stock)

Strains glycerol stock
Number Name Description Date
S1 MG1655 Standard E.Coli strain
S2 Acinetobacter ADP1 Strain used for TG coloration test with Red Nile
S3 DH5alpha pKs::DGAT Enzyme catalysing the transormation of DAG into TG
S4 pJ23100 Strong constitutive promoter in J61002 (clones 1 + 2)
S5 BBa_B0015 Double terminator (clones 1 + 2)
S6 pJ23107 Medium constitutive promoter in J61002 (clones 1 + 2) 19/07/07
S7 BBa_B0030 Strong RBS (clones 1 + 2) 19/07/07
S8 BBa_E0422 ECFP (RBS+LVA+Term) (clones 1 + 2) 19/07/07
S9 BBa_E0241 PoPs to GFP converter (clones 1 + 2) 19/07/07
S10 BBa_E0840 gfp tri-part; strong rbs (clones 1 + 2) 19/07/07
S11 BBa_J61047 Cre ORF (clones 1 + 2) 19/07/07
S12 W121 05/07/07


  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3