From 2007.igem.org

Contents 1 PCR products 2 Plasmids: MiniPrep Products 3 Digestion Products 4 Strains (glycerol stock)

PCR products

PCR products
Number	Name	Length	Comments	Product Left ?	Date
1	Lox71-FtsA-FtsZ-1	1537
2	FtsZ-2	1088
3	DGAT-1
4	DGAT-2
5	Lox66-DapAColi	985
6	Lox66-DapASubtilis	979			17/07/07
7	Lox71-FtsZ	1260	EcoR1 site not mutated		17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number	Name	Description	Product Left ?	Date
1	pJ23100 in J61002	Strong constitutive promoter in J61002 (clones 1 + 2)		15/07/07
2	pJ23107 in J61002	Medium constitutive promoter in J61002 (clones 1 + 2)		15/07/07
3	B0015 in pSB1AK3	Double terminator (clones 1 + 2)		16/07/07
4	I0500 in pSB2K3	AraC pBAD (clones 1 + 2)		16/07/07
5	BBa_B0030 in pSB1A2	Strong RBS (clones 1 + 2)		18/07/07
6	BBa_E0422 in pSB1A2	ECFP (RBS+LVA+Term) (clones 1 + 2)		18/07/07
7	BBa_E0241 in pSB1A2	PoPs to GFP converter (clones 1 + 2)		18/07/07
8	BBa_E0840 in pSB1A2	gfp tri-part; strong rbs (clones 1 + 2)		18/07/07
9	BBa_J61047 in pSB1A2	Cre ORF (clones 1 + 2)		18/07/07

Digestion Products

Digestion Products
Number	Product Name	Matrix Name	Enzyme 1	Enzyme 2	Description	Product Left	Date
D1	pJ23100		EcoR1	Spe1
D2	pJ23100		Spe1	Pst1
D3	pJ23107		EcoR1	Spe1
D4	pJ23107		Spe1	Pst1
D5	pSB2K3		EcoR1	Pst1
D6	pSB1AK3		EcoR1	Pst1
D7	Lox66 DapA E.coli (PCR product)		EcoR1	Pst1
D8	Lox66 DapA E.coli (PCR product)		Xba1	Pst1

Strains (glycerol stock)

• MG1655
• Acinetobacter ADP1
• w121
• p2C (CFP fluorescent)
• p2Y (YFP fluorescent)
• FtsZ^TS strains
  • MG1655 FtsZ -TS 84
  • 121.1
  • 121.2
  • 121.3
  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3
• DH5alpha pKs::DGAT
• pJ23100 Clone1
• pJ23100 Clone2
• BBa_B0015 Clone1
• BBa_B0015 Clone2