Paris/Freezer

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Revision as of 18:26, 18 July 2007 by David.bikard (Talk | contribs)

Contents

PCR products

PCR products
Number Name Length Comments Product Left ? Date
P1 Lox71-FtsA-FtsZ-1 1537
P2 FtsZ-2 1088
P3 DGAT-1
P4 DGAT-2
P5 Lox66-DapAColi 985
P6 Lox66-DapASubtilis 979 17/07/07
P7 Lox71-FtsZ 1260 EcoR1 site not mutated 17/07/07

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Description Product Left ? Date
MP1 pJ23100 in J61002 Strong constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP2 pJ23107 in J61002 Medium constitutive promoter in J61002 (clones 1 + 2) 15/07/07
MP3 B0015 in pSB1AK3 Double terminator (clones 1 + 2) 16/07/07
MP4 I0500 in pSB2K3 AraC pBAD (clones 1 + 2) 16/07/07
MP5 BBa_B0030 in pSB1A2 Strong RBS (clones 1 + 2) 18/07/07
MP6 BBa_E0422 in pSB1A2 ECFP (RBS+LVA+Term) (clones 1 + 2) 18/07/07
MP7 BBa_E0241 in pSB1A2 PoPs to GFP converter (clones 1 + 2) 18/07/07
MP8 BBa_E0840 in pSB1A2 gfp tri-part; strong rbs (clones 1 + 2) 18/07/07
MP9 BBa_J61047 in pSB1A2 Cre ORF (clones 1 + 2) 18/07/07

Digestion Products

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Description Product Left Date
D1 pJ23100 EcoR1 Spe1
D2 J23100 pJ23100 Spe1 Pst1
D3 pJ23107 EcoR1 Spe1
D4 pJ23107 Spe1 Pst1
D5 pSB2K3 EcoR1 Pst1
D6 pSB1AK3 EcoR1 Pst1
D7 Lox66 DapA E.coli (PCR product) EcoR1 Pst1
D8 Lox66 DapA E.coli (PCR product) Xba1 Pst1

Strains (glycerol stock)

  • MG1655
  • Acinetobacter ADP1
  • w121
  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
  • DH5alpha pKs::DGAT
  • pJ23100 Clone1
  • pJ23100 Clone2
  • BBa_B0015 Clone1
  • BBa_B0015 Clone2