From 2007.igem.org
PCR products
PCR products
|
Number
| Name
| Length
| Comments
| Product Left ?
| Date
|
P1
| Lox71-FtsA-FtsZ-1
| 1537
|
|
|
|
P2
| FtsZ-2
| 1088
|
|
|
|
P3
| DGAT-1
|
|
|
|
|
P4
| DGAT-2
|
|
|
|
|
P5
| Lox66-DapAColi
| 985
|
|
|
|
P6
| Lox66-DapASubtilis
| 979
|
|
| 17/07/07
|
P7
| Lox71-FtsZ
| 1260
| EcoR1 site not mutated
|
| 17/07/07
|
Plasmids: MiniPrep Products
Plasmids: MiniPrep Products
|
Number
| Name
| Description
| Product Left ?
| Date
|
MP1
| pJ23100 in J61002
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
| 15/07/07
|
MP2
| pJ23107 in J61002
| Medium constitutive promoter in J61002 (clones 1 + 2)
|
| 15/07/07
|
MP3
| B0015 in pSB1AK3
| Double terminator (clones 1 + 2)
|
| 16/07/07
|
MP4
| I0500 in pSB2K3
| AraC pBAD (clones 1 + 2)
|
| 16/07/07
|
MP5
| BBa_B0030 in pSB1A2
| Strong RBS (clones 1 + 2)
|
| 18/07/07
|
MP6
| BBa_E0422 in pSB1A2
| ECFP (RBS+LVA+Term) (clones 1 + 2)
|
| 18/07/07
|
MP7
| BBa_E0241 in pSB1A2
| PoPs to GFP converter (clones 1 + 2)
|
| 18/07/07
|
MP8
| BBa_E0840 in pSB1A2
| gfp tri-part; strong rbs (clones 1 + 2)
|
| 18/07/07
|
MP9
| BBa_J61047 in pSB1A2
| Cre ORF (clones 1 + 2)
|
| 18/07/07
|
Digestion Products
Digestion Products
|
Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Description
| Product Left
| Date
|
D1
| J23100
| pJ23100
| Spe1
| Pst1
| Vector with the J23100 promoter ready for insertion of a RBS/CDS insert
|
| 17/07/07
|
D2
| mRFP
| pJ23100
| Spe1
| Pst1
|
|
| 17/07/07
|
D3
| J23107
| pJ23107
| Spe1
| Pst1
| Vector with the J23107 promoter ready for insertion of a RBS/CDS insert
|
| 17/07/07
|
D4
| mRFP
| pJ23107
| Spe1
| Pst1
|
|
| 17/07/07
|
D5
| pJ23100
| pJ23100
| EcoR1
| Spe1
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 17/07/07
|
D6
| pJ23107
| pJ23107
| EcoR1
| Spe1
| The vector with the mRFP gene ready for insertion of a promoter. We are not sure the digestion worked properly.
|
| 17/07/07
|
D7
| pSB2K3
| pSB2K3::I0500
| EcoR1
| Pst1
| I'm not sure the digestion worked properly... this may not be the good fragment
|
| 17/07/07
|
D8
| pSB1AK3
| pSB1AK3::BBa_B0015
| EcoR1
| Pst1
|
|
| 17/07/07
|
D9
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| EcoR1
| Pst1
|
|
| 17/07/07
|
D10
| Lox66 DapA E.coli
| Lox66 DapA E.coli (PCR product)
| Xba1
| Pst1
|
|
| 17/07/07
|
The vector with the mRFP gene ready for insertion of a promoter
Strains (glycerol stock)
Strains glycerol stock
|
Number
| Name
| Description
| Date
|
S1
| MG1655
| Standard E.Coli strain
|
|
S2
| Acinetobacter ADP1
| Strain used for TG coloration test with Red Nile
|
|
S3
| DH5alpha pKs::DGAT
| Enzyme catalysing the transormation of DAG into TG
|
|
S4
| pJ23100
| Strong constitutive promoter in J61002 (clones 1 + 2)
|
|
S5
| BBa_B0015
| Double terminator (clones 1 + 2)
|
|
S6
| pJ23107
| Medium constitutive promoter in J61002 (clones 1 + 2)
| 19/07/07
|
S7
| BBa_B0030
| Strong RBS (clones 1 + 2)
| 19/07/07
|
S8
| BBa_E0422
| ECFP (RBS+LVA+Term) (clones 1 + 2)
| 19/07/07
|
S9
| BBa_E0241
| PoPs to GFP converter (clones 1 + 2)
| 19/07/07
|
S10
| BBa_E0840
| gfp tri-part; strong rbs (clones 1 + 2)
| 19/07/07
|
S11
| BBa_J61047
| Cre ORF (clones 1 + 2)
| 19/07/07
|
S12
| W121
|
| 05/07/07
|
- p2C (CFP fluorescent)
- p2Y (YFP fluorescent)
- FtsZTS strains
- MG1655 FtsZ -TS 84
- 121.1
- 121.2
- 121.3
- 121.4
- 121.5
- 121.6
- 129
- 1.129
- DCR 14.1
- DCR 14.2
- DCR 14.3