Paris/July 12

From 2007.igem.org

(Difference between revisions)
Line 8: Line 8:
** 450µl MgSO4
** 450µl MgSO4
** 90µl DAP
** 90µl DAP
-
Let grow until OD=0.2
+
Let grow until OD=0.2 (~1h)

Revision as of 09:41, 12 July 2007

David B:

Preparation of a stock of phage on w121:

  • Filtration of the Cacl2 and MgSO4 stocks
  • Launch a culture:
    • 15ml LB
    • 225µl Cacl2
    • 450µl MgSO4
    • 90µl DAP

Let grow until OD=0.2 (~1h)