Paris/July 12

From 2007.igem.org

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David B:
David B:
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Preparation of a stock of phage on w121:
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'''Preparation of a stock of phage on w121:'''
* Filtration of the Cacl2 and MgSO4 stocks
* Filtration of the Cacl2 and MgSO4 stocks
* Launch a culture:
* Launch a culture:

Revision as of 09:07, 12 July 2007

David B:

Preparation of a stock of phage on w121:

  • Filtration of the Cacl2 and MgSO4 stocks
  • Launch a culture:
    • 15ml LB
    • 225µl Cacl2
    • 450µl MgSO4
    • 90µl DAP

Let grow until OD=0.2