From 2007.igem.org

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Revision as of 08:59, 12 July 2007

David B:

Preparation of a stock of phage on w121:

• Filtration of the Cacl2 and MgSO4 stocks
• Launch a culture:
  • 15ml LB
  • 225µl Cacl2
  • 450µl MgSO4
  • 90µl DAP

Let grow until OD=0.2