Paris/July 12
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David.bikard (Talk | contribs) |
Nicolas C. (Talk | contribs) |
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| - | + | ==Preparation of a stock of phage on w121:== | |
* Filtration of the Cacl2 and MgSO4 stocks | * Filtration of the Cacl2 and MgSO4 stocks | ||
* Launch a culture: | * Launch a culture: | ||
| Line 17: | Line 17: | ||
* Vortex, centrifuge (15min @ 3000rpm) | * Vortex, centrifuge (15min @ 3000rpm) | ||
* Recover the supernatant = Stock keep at 4°C | * Recover the supernatant = Stock keep at 4°C | ||
| + | |||
| + | [[User:Nicolas C.|Nicolas C.]] | ||
| + | ==Titration of bacteriophages P1 == | ||
| + | We measure the strength of former P1 preparation : | ||
| + | * The stock prepared the 3.7.7 | ||
| + | * The stock prepared the 8.7.7 | ||
| + | See protocols for details. | ||
Revision as of 11:00, 12 July 2007
David B:
Preparation of a stock of phage on w121:
- Filtration of the Cacl2 and MgSO4 stocks
- Launch a culture:
- 15ml LB
- 225µl Cacl2
- 450µl MgSO4
- 90µl DAP
- 150µl W121 from an ON culture
- Let grow until OD=0.2 (~1h)
- Add 100µl P1 (stock 10^10/ml)
- Incubate 2h
- Add 1ml of the ON culture of W121
- Incubate 2h
- Add 200µl Chloroform
- Vortex, centrifuge (15min @ 3000rpm)
- Recover the supernatant = Stock keep at 4°C
Titration of bacteriophages P1
We measure the strength of former P1 preparation :
- The stock prepared the 3.7.7
- The stock prepared the 8.7.7
See protocols for details.
