Paris/July 12

From 2007.igem.org

(Difference between revisions)
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==Preparation of a stock of phage on w121:==
==Preparation of a stock of phage on w121:==
David B:   
David B:   
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==Titration of bacteriophages P1 ==
==Titration of bacteriophages P1 ==
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[[User:Nicolas C.|Nicolas C.]]
+
[[User:Nicolas C.|Nicolas C.]]<br>
We measure the strength of former P1 preparation :
We measure the strength of former P1 preparation :
* The stock prepared the 3.7.7
* The stock prepared the 3.7.7
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== Culture of FtsZ TS ==
== Culture of FtsZ TS ==
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[[User:david.bikard|David B]]
+
[[User:david.bikard|David B]]<br>
We want to isolate a good clone of the strain FstZ TS <br>
We want to isolate a good clone of the strain FstZ TS <br>
From the plate of FtsZ TS84 121: <br>
From the plate of FtsZ TS84 121: <br>
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C <br>
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C <br>
If one clone do not grow at 42, we'll use it to do the transduction
If one clone do not grow at 42, we'll use it to do the transduction

Revision as of 14:27, 12 July 2007

Preparation of a stock of phage on w121:

David B:

  • Filtration of the Cacl2 and MgSO4 stocks
  • Launch a culture:
    • 15ml LB
    • 225µl Cacl2
    • 450µl MgSO4
    • 90µl DAP
    • 150µl W121 from an ON culture
  • Let grow until OD=0.2 (~1h)
  • Add 100µl P1 (stock 10^10/ml)
  • Incubate 2h
  • Add 200µl Chloroform
  • Vortex, centrifuge (15min @ 3000rpm)
  • Recover the supernatant + add 200µl Chloroform = Stock keep at 4°C


Titration of bacteriophages P1

Nicolas C.
We measure the strength of former P1 preparation :

  • The stock prepared the 3.7.7
  • The stock prepared the 8.7.7

See protocols for details.

Culture of FtsZ TS

David B
We want to isolate a good clone of the strain FstZ TS
From the plate of FtsZ TS84 121:
isolation of 6 clones of the 121.1 on LBA+tet incubated at 42°C + Culture ON at 30°C
If one clone do not grow at 42, we'll use it to do the transduction