Paris/July 14
From 2007.igem.org
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== What to do ? == | == What to do ? == | ||
| - | * Remove the plate of the transduction experiment and those of the transformation from the 37°C incubator (Upper-left shelf): | + | * Remove the plate of the transduction experiment and those of the transformation from the 37°C incubator (Upper-left shelf): If it worked and if someones comes Sunday: |
| - | If it worked and if someones comes Sunday: | + | |
** isolation of clones of the transduction on petri-dishes (LB+DAP+Erm+Citrate) | ** isolation of clones of the transduction on petri-dishes (LB+DAP+Erm+Citrate) | ||
** LB+ antibio culture of the transformants (to make a glycerol stock and to do MiniPreps) | ** LB+ antibio culture of the transformants (to make a glycerol stock and to do MiniPreps) | ||
Revision as of 17:33, 13 July 2007
What to do ?
- Remove the plate of the transduction experiment and those of the transformation from the 37°C incubator (Upper-left shelf): If it worked and if someones comes Sunday:
- isolation of clones of the transduction on petri-dishes (LB+DAP+Erm+Citrate)
- LB+ antibio culture of the transformants (to make a glycerol stock and to do MiniPreps)
- Make a gel (0.8%) and migrate the PCR products
- Purify them
If enough time:
- Digestion of products with appropriate enzymes
- Purification
