Paris/July 14

From 2007.igem.org

(Difference between revisions)
(What to do ?)
(What to do ?)
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* Digestion of the purifications products with appropriate enzymes
* Digestion of the purifications products with appropriate enzymes
* Purification
* Purification
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== What has been done ==
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* Several colonies of transduced MG1655 are obtained (transduction from dapA- strain w121):
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** Isolation of 10 different colonies on LB Citrate Erythro DAP plates

Revision as of 09:39, 14 July 2007

What to do ?

  • Remove the plate of the transduction experiment and the transformation ones from the 37°C incubator (Upper-left shelf): If it has worked and if someones comes on Sunday:
    • isolation of the clones from the transduction on petri-dishes (LB+DAP+Erm+Citrate)
    • LB + antibio culture of the transformants (to make a glycerol stock and to do MiniPreps)
  • Make a gel (0.8%) and migrate the PCR products
  • Purify them
  • Do the assembly PCR

If enough time (we can also wait to have the plasmids to do everything at the same time):

  • Digestion of the purifications products with appropriate enzymes
  • Purification


What has been done

  • Several colonies of transduced MG1655 are obtained (transduction from dapA- strain w121):
    • Isolation of 10 different colonies on LB Citrate Erythro DAP plates