Paris/July 14
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== What to do ? == | == What to do ? == | ||
| - | * Remove the plate of the transduction experiment and | + | * Remove the plate of the transduction experiment and the transformation ones from the 37°C incubator (Upper-left shelf): If it has worked and if someones comes on Sunday: |
| - | ** isolation of clones | + | ** isolation of the clones from the transduction on petri-dishes (LB+DAP+Erm+Citrate) |
| - | ** LB+ antibio culture of the transformants (to make a glycerol stock and to do MiniPreps) | + | ** LB + antibio culture of the transformants (to make a glycerol stock and to do MiniPreps) |
* Make a gel (0.8%) and migrate the PCR products | * Make a gel (0.8%) and migrate the PCR products | ||
Revision as of 17:37, 13 July 2007
What to do ?
- Remove the plate of the transduction experiment and the transformation ones from the 37°C incubator (Upper-left shelf): If it has worked and if someones comes on Sunday:
- isolation of the clones from the transduction on petri-dishes (LB+DAP+Erm+Citrate)
- LB + antibio culture of the transformants (to make a glycerol stock and to do MiniPreps)
- Make a gel (0.8%) and migrate the PCR products
- Purify them
If enough time (we can also wait to have the plasmids to do everything at the same time):
- Digestion of the purifications products with appropriate enzymes
- Purification
