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Revision as of 16:44, 18 July 2007 (view source) Landrain (Talk | contribs) ← Older edit		Revision as of 16:45, 18 July 2007 (view source) Landrain (Talk | contribs) (→Minipreps) Newer edit →
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	== Minipreps==		== Minipreps==
	We have 2 clones for each plasmid :		We have 2 clones for each plasmid :
-	<br>
	* E0422		* E0422
	* E0241		* E0241
Line 24:		Line 23:
	* B0030		* B0030
	* J61047		* J61047
-	<br>	+	Elution within 50µL H2O
-	DNA is within the Miniprep box in the -20°C fridge	+	DNA is within the Miniprep box in the -20°C fridge.

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Revision as of 16:45, 18 July 2007

FtsZ^TS strain screening

We want to test these strains :

• 121.4
• 121.5
• 121.6
• 129
• 1.129
• DCR 14.1
• DCR 14.2
• DCR 14.3

I made glycerol stock of these strains, stored in the freezer. Test :

• Spread these strains on two plates (preheated before spreading) :
  • One at 30°C
  • One at 42°C

The results tomorrow.

Minipreps

We have 2 clones for each plasmid :

• E0422
• E0241
• E0840
• B0030
• J61047

Elution within 50µL H2O DNA is within the Miniprep box in the -20°C fridge.