Paris/July 19

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Revision as of 21:37, 19 July 2007 by Eismoustique (Talk | contribs)

FtsZTS strain screening

We want to test these strains :

  • 121.4
  • 121.5
  • 121.6
  • 129
  • 1.129
  • DCR 14.1
  • DCR 14.2
  • DCR 14.3

I made glycerol stock of these strains, stored in the freezer. Test :

  • Spread these strains on two plates (preheated before spreading) :
    • One at 30°C
    • One at 42°C

Results : every strain grew at 30 and 42°C => fail. >br >br

ON Bacterial cultures

We launched several cultures :

  • MG1655 -> to do transductions tomorrow (don't forget the DAP, David!)
  • pJ23107_J61002 clones 3 & 4 in LB-Amp liquid; also striping clones 3-18 on LB-Amp Agar plates
  • I0500_pSB2K3 clones 1 & 2 in LB-Kan liquid medium
  • B0015_pSB1AK3 clones 3 & 4 in LB-Kan liquid medium

Tomorrow (20/07/07): perform transduction (MG1655), minipreps, glycerol stocks