Paris/July 20

From 2007.igem.org

(Difference between revisions)
(PCRs)
Line 17: Line 17:
|DNA= MP3_pSB1AK3-BBa_B0015 0.5µl
|DNA= MP3_pSB1AK3-BBa_B0015 0.5µl
|Size= 1084
|Size= 1084
-
|Success=
+
|Success= ?
|Image=
|Image=
|Band=
|Band=
Line 37: Line 37:
|DNA= toothpick in MG1655 glycerol
|DNA= toothpick in MG1655 glycerol
|Size= 2588
|Size= 2588
-
|Success=
+
|Success= ? The PCR seemed to have worked but the purification failed
|Image=
|Image=
|Band=
|Band=

Revision as of 22:54, 23 July 2007

PCRs

PCR : Lox71-KmR-Lox66
PCR Settings Buffer (5x) 5x 10µL Expected size
Annealing (°C) MgCl2 10µM 10µM 0µL 1084
55 dNTP 10µM 10µM 1µL Success
Time Elongation Oligo F 10µM 10 Lox71-KmR-F 2.5µL  ?
3m00' Oligo R 10µM 11 Lox66-KmR-R 2.5µL Image (click to enlarge)
Number cycles Water 34µl [[Image:|30px]]
35 Polymerase Phusion 0.5µL Band (0=ladder)
DNA MP3_pSB1AK3-BBa_B0015 0.5µl
PCR : Lox71-FtsA-FtsZ
PCR Settings Buffer (5x) 5x 10µL Expected size
Annealing (°C) MgCl2 10µM 10µM 0µL 2588
55 dNTP 10µM 10µM 1µL Success
Time Elongation Oligo F 10µM 3 Lox71-FtsA-F 2.5µL  ? The PCR seemed to have worked but the purification failed
3m00' Oligo R 10µM 2 FtsZ-R 2.5µL Image (click to enlarge)
Number cycles Water 34µl [[Image:|30px]]
35 Polymerase Phusion 0.5µL Band (0=ladder)
DNA toothpick in MG1655 glycerol

Glycerol stock

  • BBa_pJ23107 clone 3&4 (S14)
  • BBa_I0500 clone 1&2 (S13)

Minipreps on Biobricks

  • BBa_pJ23107 clone 3&4
  • BBa_I0500 clone 1&2