Paris/July 21

From 2007.igem.org

(Difference between revisions)
(Digestion reactions)
(Digestion reactions)
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|EcoI
|EcoI
|SpeI
|SpeI
-
|
+
|2096
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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|EcoI
|EcoI
|SpeI
|SpeI
-
|
+
|58
-
|J23100 promoter ready for usage as a fw-insert
+
|J23100 promoter ready for use as a fw-insert
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
| style="background: #ccffcc;" |D11
| style="background: #ccffcc;" |D11
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|EcoRI
|EcoRI
|SpeI
|SpeI
-
|
+
|2096
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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|EcoRI
|EcoRI
|SpeI
|SpeI
-
|
+
|58
|J23107 promoter ready for usage as a fw-insert
|J23107 promoter ready for usage as a fw-insert
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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|SpeI
|SpeI
|PstI
|PstI
-
|
+
|887
|mRFP RBS/CDS/T sequence, can it be used as a backward inser?
|mRFP RBS/CDS/T sequence, can it be used as a backward inser?
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   

Revision as of 14:27, 23 July 2007

Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D9 pJ23100 ES vector pJ23100 (MP1.1 & MP1.2) EcoI SpeI 2096 Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert)
D10 pJ23100 promoter fw-insert pJ23100 (MP1.1 & MP1.2) EcoI SpeI 58 J23100 promoter ready for use as a fw-insert
D11 pJ23107 ES vector pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 2096 Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter
D12 pJ23107 promoter fw-insert pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) EcoRI SpeI 58 J23107 promoter ready for usage as a fw-insert
D13 mRFP pJ23107 (MP2.2, MP2.3 & MP2.4) SpeI PstI 887 mRFP RBS/CDS/T sequence, can it be used as a backward inser?
D14 Cre ORF J61047 MP9.1 & MP9.2 XbaI PstI Cre ORF extracted for usage as a bw-insert
D15 B0030 bw-vector B0030 MP5.1 & MP5.2 SpeI PstI bacward vector for cloning a CDS bw-insert after the B0030 RBS
D16 pSB1AK3 vector B0015 MP3.1 & MP3.2 EcoRI PstI pSB1AK3 open vector for BioBrick cloning
D17 pSB1A2 E0422 vector E0422 (MP6.1 & MP6.2) EcoRI PstI pSB1A2 open vector for BioBrick cloning
D18 BB dig. lox66DapAE.coli lox66-DapAE.coli P5 PCR product EcoRI PstI PCR product digested for cloning as a BioBrick
D19 BB dig. lox66DapAsubtilis lox66-DapAsubtilis P6 PCR product EcoRI PstI PCR product digested for cloning as a BioBrick
D20 fw insert lox66-DapAE.coli lox66-DapAE.coli P5 PCR product XbaI PstI PCR product digested for usage as a forward insert
D21 fw insert lox66-DapAsubtilis lox66-DapAsubtilis P6 PCR product XbaI PstI PCR product digested for usage as a forward insert