Paris/July 21
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David.bikard (Talk | contribs) (→Digestion reactions) |
David.bikard (Talk | contribs) (→Digestion reactions) |
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|2096 | |2096 | ||
|Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | |Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D10 | | style="background: #ccffcc;" |D10 | ||
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|58 | |58 | ||
|J23100 promoter ready for use as a fw-insert | |J23100 promoter ready for use as a fw-insert | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D11 | | style="background: #ccffcc;" |D11 | ||
| Line 35: | Line 39: | ||
|2096 | |2096 | ||
|Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | |Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D12 | | style="background: #ccffcc;" |D12 | ||
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|58 | |58 | ||
|J23107 promoter ready for usage as a fw-insert | |J23107 promoter ready for usage as a fw-insert | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D13 | | style="background: #ccffcc;" |D13 | ||
| Line 50: | Line 58: | ||
|PstI | |PstI | ||
|887 | |887 | ||
| - | |mRFP RBS/CDS/T sequence, can it be used as a backward | + | |mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to any biobrick after it |
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D14 | | style="background: #ccffcc;" |D14 | ||
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|XbaI | |XbaI | ||
|PstI | |PstI | ||
| - | | | + | |1063 |
|Cre ORF extracted for usage as a bw-insert | |Cre ORF extracted for usage as a bw-insert | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D15 | | style="background: #ccffcc;" |D15 | ||
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|bacward vector for cloning a CDS bw-insert after the B0030 RBS | |bacward vector for cloning a CDS bw-insert after the B0030 RBS | ||
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D16 | | style="background: #ccffcc;" |D16 | ||
| Line 75: | Line 89: | ||
| | | | ||
|pSB1AK3 open vector for BioBrick cloning | |pSB1AK3 open vector for BioBrick cloning | ||
| + | | | ||
| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D17 | | style="background: #ccffcc;" |D17 | ||
| Line 83: | Line 99: | ||
| | | | ||
|pSB1A2 open vector for BioBrick cloning | |pSB1A2 open vector for BioBrick cloning | ||
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D18 | | style="background: #ccffcc;" |D18 | ||
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| | | | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D19 | | style="background: #ccffcc;" |D19 | ||
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| | | | ||
|PCR product digested for cloning as a BioBrick | |PCR product digested for cloning as a BioBrick | ||
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D20 | | style="background: #ccffcc;" |D20 | ||
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| | | | ||
|PCR product digested for usage as a forward insert | |PCR product digested for usage as a forward insert | ||
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| + | | | ||
|- style="background: #cccccc;" | |- style="background: #cccccc;" | ||
| style="background: #ccffcc;" |D21 | | style="background: #ccffcc;" |D21 | ||
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|PCR product digested for usage as a forward insert | |PCR product digested for usage as a forward insert | ||
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Revision as of 14:30, 23 July 2007
Digestion reactions
| Digestion Products | ||||||||
|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | size | Description | ||
| D9 | pJ23100 ES vector | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 2096 | Vector with mRFP RBS/CDS ready for insertion promoter (fw-insert) | ||
| D10 | pJ23100 promoter fw-insert | pJ23100 (MP1.1 & MP1.2) | EcoI | SpeI | 58 | J23100 promoter ready for use as a fw-insert | ||
| D11 | pJ23107 ES vector | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 2096 | Vector with mRFP RBS/CDS ready for insertion of a fw-insert promoter | ||
| D12 | pJ23107 promoter fw-insert | pJ23107 (MP2.1, MP2.2, MP2.3 & MP2.4) | EcoRI | SpeI | 58 | J23107 promoter ready for usage as a fw-insert | ||
| D13 | mRFP | pJ23107 (MP2.2, MP2.3 & MP2.4) | SpeI | PstI | 887 | mRFP RBS/CDS/T sequence, can it be used as a backward insert? => yes but you will not be able to any biobrick after it | ||
| D14 | Cre ORF | J61047 MP9.1 & MP9.2 | XbaI | PstI | 1063 | Cre ORF extracted for usage as a bw-insert | ||
| D15 | B0030 bw-vector | B0030 MP5.1 & MP5.2 | SpeI | PstI | bacward vector for cloning a CDS bw-insert after the B0030 RBS | |||
| D16 | pSB1AK3 vector | B0015 MP3.1 & MP3.2 | EcoRI | PstI | pSB1AK3 open vector for BioBrick cloning | |||
| D17 | pSB1A2 E0422 vector | E0422 (MP6.1 & MP6.2) | EcoRI | PstI | pSB1A2 open vector for BioBrick cloning | |||
| D18 | BB dig. lox66DapAE.coli | lox66-DapAE.coli P5 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |||
| D19 | BB dig. lox66DapAsubtilis | lox66-DapAsubtilis P6 PCR product | EcoRI | PstI | PCR product digested for cloning as a BioBrick | |||
| D20 | fw insert lox66-DapAE.coli | lox66-DapAE.coli P5 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |||
| D21 | fw insert lox66-DapAsubtilis | lox66-DapAsubtilis P6 PCR product | XbaI | PstI | PCR product digested for usage as a forward insert | |||
