Paris/July 22
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| + | [[Paris/July 21|yesterday]] -- [[Paris/July 23|tomorrow]] <br> | ||
== Digestion products == | == Digestion products == | ||
Purification of the digestion products of 21/07/07 | Purification of the digestion products of 21/07/07 | ||
| + | purif. using the SV wizard gel cleanup system | ||
| - | == | + | elution of the purification products in 30-40microL H2O |
| + | |||
| + | == Overnight cultures launched == | ||
The following ON cultures were launched: | The following ON cultures were launched: | ||
| - | |||
*pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep | *pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep | ||
| - | *w121 (from glycerol stock S12) in LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media | + | *w121 (from glycerol stock S12) in 5 ml LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media |
| + | |||
| + | I was unable to launch the following culture because of lack of a glycerol stock: | ||
| + | *pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep | ||
| + | |||
| + | == E.Coli pKS::DGAT == | ||
| + | |||
| + | We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See [[Paris/July_18#E.coli_pKS::DGAT|July 18]]). | ||
| + | |||
| + | * Observation: | ||
| + | We can observe E.coli single cells (100X). | ||
| + | [[Image: coli_dgat_07222007.jpg|center|900px]] | ||
| + | |||
| + | |||
| + | * Interpretation: | ||
| + | |||
| + | We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences. Signal would be better with more cells. | ||
Latest revision as of 17:49, 7 October 2007
Digestion products
Purification of the digestion products of 21/07/07 purif. using the SV wizard gel cleanup system
elution of the purification products in 30-40microL H2O
Overnight cultures launched
The following ON cultures were launched:
- pJ23107 clone 3 (from the glycerol stock S14.3) in 5ml LB-Amp. For performing a miniprep
- w121 (from glycerol stock S12) in 5 ml LB-Erythromycin. For performing growth-kinetics analysis on DAP supplemented media
I was unable to launch the following culture because of lack of a glycerol stock:
- pJ23107 clone 2 (from the glycerol stock S6.2) in 5ml LB-Amp. For performing a miniprep
E.Coli pKS::DGAT
We look under microscopy 72 hours after incubation of E.coli transformed by pKS::DGAT and the control E.coli transformed by part B0015 on different LB medium (See July 18).
- Observation:
We can observe E.coli single cells (100X).
- Interpretation:
We can observe lipid inclusion into E.coli transformed by pKS::DGAT with IPTG induction. With or without adding oleate we do not observe significant differences. Signal would be better with more cells.
