Paris/July 23

From 2007.igem.org

(Difference between revisions)
(Transduction of DapA deletion in MG1655)
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== w121 growth ==
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w121 culture launched on 22/07/07 did not grow ON: I forgot to add DAP to LB-Erythro growth medium.
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at 12h30, the following culture was launched:
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*w121 in 2ml LB-Erythro-DAP
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== Transduction of DapA deletion in MG1655 ==
== Transduction of DapA deletion in MG1655 ==

Revision as of 13:30, 23 July 2007

Contents

w121 growth

w121 culture launched on 22/07/07 did not grow ON: I forgot to add DAP to LB-Erythro growth medium.

at 12h30, the following culture was launched:

  • w121 in 2ml LB-Erythro-DAP

Transduction of DapA deletion in MG1655

  • The transduction seems to have worked: isolation of clones on LB and LB+DAP for verification.

Migration of digestion products

MiniPrep

Anealing of lox66 and lox71

Ligations and transformations