Paris/July 23

From 2007.igem.org

(Difference between revisions)
(→w121 growth)
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== Anealing of lox66 and lox71 ==
== Anealing of lox66 and lox71 ==
-
== Ligations and transformations==
+
== Digestion reactions ==
 +
 
 +
{|
 +
|- style="background: #ccccff;"
 +
! colspan="5" style="background: #ccccff;" | Digestion Products
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=20%| Product Name
 +
|width=30%| Matrix Name
 +
|width=5%| Enzyme 1
 +
|width=5%| Enzyme 2
 +
|width=5%| size
 +
|width=35%| Description
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D22
 +
|pSB1A2 open vector
 +
|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
 +
|EcoI
 +
|PstI
 +
|
 +
|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D23
 +
|AraC/pBad promoter fw-insert
 +
|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
 +
|EcoI
 +
|SpeI
 +
|
 +
|AraC/pBad promoter ready for use as a forward insert
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D24
 +
|lox71-ftsZ bw-insert
 +
|lox71-ftsZ PCR product P7
 +
|XbaI
 +
|PstI
 +
|
 +
|lox71-ftsZ(unmutated) ready for use as a backward insert
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D25
 +
|eCFP bw-insert
 +
|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
 +
|XbaI
 +
|PstI
 +
|
 +
|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D26
 +
|PoPs to GFP converter
 +
|<bbpart>BBa_E0421</bbpart> (MP7.1 & MP7.2)
 +
|XbaI
 +
|PstI
 +
|
 +
|PoPs to GFP converter ready for use as a backward insert
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |D27
 +
|gfp-tripart
 +
|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
 +
|XbaI
 +
|PstI
 +
|
 +
|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
 +
|
 +
|
 +
|}

Revision as of 22:38, 23 July 2007

Contents

w121 growth

w121 culture launched on 22/07/07 did not grow ON: I had forgot to add DAP to LB-Erythro growth medium.

at 12h30, the following culture was launched:

  • w121 in 2ml LB-Erythro-DAP

Transduction of DapA deletion in MG1655

  • The transduction seems to have worked: isolation of clones on LB and LB+DAP for verification.

Migration of digestion products

MiniPrep

Anealing of lox66 and lox71

Digestion reactions

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D22 pSB1A2 open vector BBa_J61047 (MP9.1 & MP9.2) EcoI PstI Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
D23 AraC/pBad promoter fw-insert BBa_I0500 (MP4.1 & MP4.2) EcoI SpeI AraC/pBad promoter ready for use as a forward insert
D24 lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert
D25 eCFP bw-insert BBa_E0422 (MP6.1 & MP6.2) XbaI PstI eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
D26 PoPs to GFP converter BBa_E0421 (MP7.1 & MP7.2) XbaI PstI PoPs to GFP converter ready for use as a backward insert
D27 gfp-tripart BBa_E0840 (MP8.1 & MP8.2) XbaI PstI gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
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