Paris/July 23

From 2007.igem.org

(Difference between revisions)
(Digestion reactions)
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== Digestion reactions ==
== Digestion reactions ==
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Each of the digestion reactions that follow were performed as follows:
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*20µL DNA solution (miniprep or PCR product)
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*5µL Buffer 10x
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*0.5µL BSA 100x
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*2µL of each of the 2 enzymes indicated
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*H20 qsp 50µL
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NEB3 buffer used for XbaI/PstI double digestion
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NEBEcoRI buffer used for both EcoRI/SpeI & EcoRI/PstI double digestion reactions
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Revision as of 22:50, 23 July 2007

Contents

w121 growth

w121 culture launched on 22/07/07 did not grow ON: I had forgot to add DAP to LB-Erythro growth medium.

at 12h30, the following culture was launched:

  • w121 in 2ml LB-Erythro-DAP

Transduction of DapA deletion in MG1655

  • The transduction seems to have worked: isolation of clones on LB and LB+DAP for verification.

Migration of digestion products

MiniPrep

Anealing of lox66 and lox71

Digestion reactions

Each of the digestion reactions that follow were performed as follows:

  • 20µL DNA solution (miniprep or PCR product)
  • 5µL Buffer 10x
  • 0.5µL BSA 100x
  • 2µL of each of the 2 enzymes indicated
  • H20 qsp 50µL

NEB3 buffer used for XbaI/PstI double digestion NEBEcoRI buffer used for both EcoRI/SpeI & EcoRI/PstI double digestion reactions


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D22 pSB1A2 open vector BBa_J61047 (MP9.1 & MP9.2) EcoI PstI Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
D23 AraC/pBad promoter fw-insert BBa_I0500 (MP4.1 & MP4.2) EcoI SpeI AraC/pBad promoter ready for use as a forward insert
D24 lox71-ftsZ bw-insert lox71-ftsZ PCR product P7 XbaI PstI lox71-ftsZ(unmutated) ready for use as a backward insert
D25 eCFP bw-insert BBa_E0422 (MP6.1 & MP6.2) XbaI PstI eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
D26 PoPs to GFP converter BBa_E0421 (MP7.1 & MP7.2) XbaI PstI PoPs to GFP converter ready for use as a backward insert
D27 gfp-tripart BBa_E0840 (MP8.1 & MP8.2) XbaI PstI gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert