From 2007.igem.org

Contents 1 w121 growth 2 Transduction of DapA deletion in MG1655 3 Migration of digestion products 4 MiniPrep 5 Anealing of lox66 and lox71 6 Ligations and transformations

w121 growth

w121 culture launched on 22/07/07 did not grow ON: I had forgot to add DAP to LB-Erythro growth medium.

at 12h30, the following culture was launched:

• w121 in 2ml LB-Erythro-DAP

Transduction of DapA deletion in MG1655

• The transduction seems to have worked: isolation of clones on LB and LB+DAP for verification.

Migration of digestion products

MiniPrep

• BBa_J23107

Anealing of lox66 and lox71

Ligations and transformations