Paris/July 25

From 2007.igem.org

(Difference between revisions)
(=Digestions)
(=Digestions)
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|width=35%| Description
|width=35%| Description
|- style="background: #cccccc;"   
|- style="background: #cccccc;"   
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| style="background: #ccffcc;" |D22
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| style="background: #ccffcc;" |D ?
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|pSB1A2 open vector
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|RBS-DapAColi BB
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|<bbpart>BBa_J61047</bbpart> (MP9.1 & MP9.2)
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|P8 (RBS-DapAColi)
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|EcoI
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|EcoRI
|PstI
|PstI
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|Open pSB1A2 vector for BioBrick EcoRI/PstI insertio
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D23
 
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|AraC/pBad promoter fw-insert
 
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|<bbpart>BBa_I0500</bbpart> (MP4.1 & MP4.2)
 
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|EcoI
 
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|SpeI
 
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|
 
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|AraC/pBad promoter ready for use as a forward insert
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D24
 
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|eCFP bw-insert
 
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|<bbpart>BBa_E0422</bbpart> (MP6.1 & MP6.2)
 
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|XbaI
 
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|PstI
 
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|eCFP (RBS-OFR_LVA-T) ready for use as a backward insert
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D25
 
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|PoPs to GFP converter
 
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|<bbpart>BBa_E0421</bbpart> (MP7.1 & MP7.2)
 
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|XbaI
 
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|PstI
 
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|PoPs to GFP converter ready for use as a backward insert
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D26
 
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|gfp-tripart
 
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|<bbpart>BBa_E0840</bbpart> (MP8.1 & MP8.2)
 
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|XbaI
 
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|PstI
 
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|gfp-tri part (strongRBS-ORF-T) ready for use as a backward insert
 
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|- style="background: #cccccc;" 
 
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| style="background: #ccffcc;" |D27
 
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|lox71-ftsZ bw-insert
 
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|lox71-ftsZ PCR product P7
 
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|XbaI
 
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|PstI
 
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|lox71-ftsZ(unmutated) ready for use as a backward insert
 
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Revision as of 10:36, 25 July 2007

=Digestions

  • P8 (RBS-DapAColi) with XbaI & PstI for BW insert
                                      EcoRI & PstI for BioBricks
  • P9 (RBS-DapASubtilis) with XbaI & PstI for BW insert
                                            EcoRI & PstI for BioBricks
  • P10 (Lox71-FtsA-FtsZ) with XbaI & PstI for BW insert


Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D ? RBS-DapAColi BB P8 (RBS-DapAColi) EcoRI PstI