Paris/July 25

From 2007.igem.org

(Difference between revisions)
(Digestions)
(Digestions)
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|PstI
|PstI
|~2600
|~2600
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|Backward insert - EcoRI site still presents : don't have to use EcoRI
+
|Backward insert - EcoRI site still presents : we can't use EcoRI
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Revision as of 15:20, 25 July 2007

Digestions

Digestion of purifications of PCR products

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 size Description
D28 RBS-DapAColi BB P8 (RBS-DapAColi) EcoRI PstI ~950 Biobrick
D29 RBS-DapAColi P8 (RBS-DapAColi) XbaI PstI ~950 Backward insert
D30 RBS-DapASubtilis BB P9 (RBS-DapASubtilis) EcoRI PstI ~950 Biobrick
D31 RBS-DapASubtilis P9 (RBS-DapASubtilis) XbaI PstI ~950 Backward insert
D32 Lox71-FtsA-FtsZ P10 Lox71-FtsA-FtsZ XbaI PstI ~2600 Backward insert - EcoRI site still presents : we can't use EcoRI