Paris/July 26
From 2007.igem.org
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| - | Colonies PCR for cloning verification | + | == Colonies PCR for cloning verification == |
L1.1, L1.2, L2.1, L2.2, L9.1, L10.1 | L1.1, L1.2, L2.1, L2.2, L9.1, L10.1 | ||
| Line 18: | Line 18: | ||
*60°C 30'' | *60°C 30'' | ||
*68°C 2' | *68°C 2' | ||
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| + | == Auxotrophy microscopy experiment == | ||
Revision as of 14:08, 31 July 2007
Colonies PCR for cloning verification
L1.1, L1.2, L2.1, L2.2, L9.1, L10.1
L6: 8 clones
Toothpick in 50µl sterile water ==> 10'@100°C
PCR Mix:
- 212.5 µl Master Mix
- 8.5 µl VF2
- 8.5 µl VR
12 µl of the mix in 13µl of lysed cells for each PCR reaction
Cycle x30:
- 95°C 30
- 60°C 30
- 68°C 2'
