Paris/July 26

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Colonies PCR for cloning verification
Colonies PCR for cloning verification
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 +
L1.1, L1.2, L2.1, L2.2, L9.1, L10.1
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L6: 8 clones
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 +
Toothpick in 50µl sterile water ==> 10'@100°C
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 +
'''PCR Mix:'''
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* 212.5 µl Master Mix
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* 8.5 µl VF2
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* 8.5 µl VR
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*12 µl of the mix in 13µl of lysed cells
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 +
'''Cycle x30:'''
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*95°C 30''
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*60°C 30''
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*68°C 2'

Revision as of 16:26, 27 July 2007

Colonies PCR for cloning verification

L1.1, L1.2, L2.1, L2.2, L9.1, L10.1

L6: 8 clones

Toothpick in 50µl sterile water ==> 10'@100°C

PCR Mix:

  • 212.5 µl Master Mix
  • 8.5 µl VF2
  • 8.5 µl VR
  • 12 µl of the mix in 13µl of lysed cells

Cycle x30:

  • 95°C 30
  • 60°C 30
  • 68°C 2'