Paris/July 26
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David.bikard (Talk | contribs) |
David.bikard (Talk | contribs) |
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* 8.5 µl VR | * 8.5 µl VR | ||
| - | *12 µl of the mix in 13µl of lysed cells | + | **12 µl of the mix in 13µl of lysed cells |
'''Cycle x30:''' | '''Cycle x30:''' | ||
Revision as of 16:26, 27 July 2007
Colonies PCR for cloning verification
L1.1, L1.2, L2.1, L2.2, L9.1, L10.1
L6: 8 clones
Toothpick in 50µl sterile water ==> 10'@100°C
PCR Mix:
- 212.5 µl Master Mix
- 8.5 µl VF2
- 8.5 µl VR
- 12 µl of the mix in 13µl of lysed cells
Cycle x30:
- 95°C 30
- 60°C 30
- 68°C 2'
